Results

RDF/XMLNTriplesTurtleShow queryShare
SubjectPredicateObject
http://purl.uniprot.org/citations/17958744http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17958744http://www.w3.org/2000/01/rdf-schema#comment"

Background

A functional role for several components of the fibrinolytic (plasminogen/plasmin) system in development of adipose tissue has been demonstrated. No information is available, however, on a potential role of plasminogen activator inhibitor-2 (PAI-2) in obesity.

Methods

In vitro, 3T3-F442A murine pre-adipocytes were differentiated into mature adipocytes. In vivo, 5-week-old male PAI-2-deficient (PAI-2(-/-)) mice and wild-type (WT) controls of the same genetic background (C57Bl/6) were kept on a high fat diet (HFD, caloric value of 20.1 kJ g(-1)) for 15 weeks.

Results

Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) revealed expression of PAI-2 mRNA during in vitro differentiation of pre-adipocytes and in vivo in s.c. and gonadal (GON) adipose tissues of WT mice, where it was localized both in the stromal/vascular cell fraction and in adipocytes. During HFD feeding, food intake and body weight gain were comparable for WT and PAI-2(-/-) mice. Subcutaneous plus GON fat mass was, however, significantly lower in PAI-2(-/-) mice (3.15 +/- 0.21 vs. 3.91 +/-0.18 g; P < 0.05). Immunohistochemical analysis of adipose tissues revealed significant adipocyte hypotrophy in s.c. fat of PAI-2(-/-) mice (about 25% reduction in size; P < 0.01). Blood vessel density, normalized to adipocyte number, was comparable in s.c. fat, but was lower (P < 0.05) in GON fat of PAI-2(-/-) mice. Adipose tissue-associated fibrinolytic activity was not affected by PAI-2 deficiency.

Conclusion

PAI-2 promotes adipose tissue development in mice via a mechanism independent of its antifibrinolytic effect."xsd:string
http://purl.uniprot.org/citations/17958744http://purl.org/dc/terms/identifier"doi:10.1111/j.1538-7836.2007.02735.x"xsd:string
http://purl.uniprot.org/citations/17958744http://purl.uniprot.org/core/author"Lijnen H.R."xsd:string
http://purl.uniprot.org/citations/17958744http://purl.uniprot.org/core/author"Frederix L."xsd:string
http://purl.uniprot.org/citations/17958744http://purl.uniprot.org/core/author"Scroyen I."xsd:string
http://purl.uniprot.org/citations/17958744http://purl.uniprot.org/core/date"2007"xsd:gYear
http://purl.uniprot.org/citations/17958744http://purl.uniprot.org/core/name"J Thromb Haemost"xsd:string
http://purl.uniprot.org/citations/17958744http://purl.uniprot.org/core/pages"2259-2265"xsd:string
http://purl.uniprot.org/citations/17958744http://purl.uniprot.org/core/title"Deficiency of plasminogen activator inhibitor-2 impairs nutritionally induced murine adipose tissue development."xsd:string
http://purl.uniprot.org/citations/17958744http://purl.uniprot.org/core/volume"5"xsd:string
http://purl.uniprot.org/citations/17958744http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/17958744
http://purl.uniprot.org/citations/17958744http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/17958744
http://purl.uniprot.org/uniprot/#_F6QN99-mappedCitation-17958744http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17958744
http://purl.uniprot.org/uniprot/#_F6ZFV0-mappedCitation-17958744http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17958744
http://purl.uniprot.org/uniprot/#_P12388-mappedCitation-17958744http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17958744
http://purl.uniprot.org/uniprot/#_Q542A3-mappedCitation-17958744http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17958744
http://purl.uniprot.org/uniprot/F6QN99http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/17958744
http://purl.uniprot.org/uniprot/F6ZFV0http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/17958744
http://purl.uniprot.org/uniprot/P12388http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/17958744
http://purl.uniprot.org/uniprot/Q542A3http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/17958744