| http://purl.uniprot.org/citations/17964421 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17964421 | http://www.w3.org/2000/01/rdf-schema#comment | "Chronic ethanol consumption causes increased production of reactive oxygen species in hepatic mitochondria accompanied by elevations in products of lipid peroxidation such as 4-hydroxynonenal (4-HNE). In the current study we investigated the effects of chronic ethanol consumption on a prominent protein-4-HNE adduct in liver mitochondria. Male Sprague-Dawley rats were fed a liquid diet for 31 days in which ethanol constituted 36% of total calories. Immunoblot analyses of liver mitochondria from ethanol-fed and control animals, using an antibody to a 4-HNE-protein adduct, demonstrated elevated 4-HNE binding (+50%) to a mitochondrial protein of approximately 55 kDa due to chronic ethanol consumption. Analysis of this protein using AspN digestion and tandem mass spectrometry identified it as the mitochondrial form of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase. Activity of the activated form of this enzyme was unchanged in livers from ethanol-fed animals, but the protein level was elevated by 36%, which suggests a compensatory mechanism to maintain constant levels of synthase activity in the mitochondrion in the face of continuous inactivation by 4-HNE. Treatment of isolated mitochondria with 4-HNE demonstrated that the enzyme activity decreased as a function of 4-HNE concentration and with time of exposure. This study demonstrates that ethanol consumption increases the formation of a 4-HNE adduct with mitochondrial HMG-CoA synthase, which has the potential to inactivate the enzyme in situ."xsd:string |
| http://purl.uniprot.org/citations/17964421 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.freeradbiomed.2007.08.004"xsd:string |
| http://purl.uniprot.org/citations/17964421 | http://purl.uniprot.org/core/author | "Patel V.B."xsd:string |
| http://purl.uniprot.org/citations/17964421 | http://purl.uniprot.org/core/author | "Lively M.O."xsd:string |
| http://purl.uniprot.org/citations/17964421 | http://purl.uniprot.org/core/author | "Young T.A."xsd:string |
| http://purl.uniprot.org/citations/17964421 | http://purl.uniprot.org/core/author | "Cunningham C.C."xsd:string |
| http://purl.uniprot.org/citations/17964421 | http://purl.uniprot.org/core/author | "Spencer C.H."xsd:string |
| http://purl.uniprot.org/citations/17964421 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17964421 | http://purl.uniprot.org/core/name | "Free Radic Biol Med"xsd:string |
| http://purl.uniprot.org/citations/17964421 | http://purl.uniprot.org/core/pages | "1499-1507"xsd:string |
| http://purl.uniprot.org/citations/17964421 | http://purl.uniprot.org/core/title | "Effects of 4-hydroxynonenal on mitochondrial 3-hydroxy-3-methylglutaryl (HMG-CoA) synthase."xsd:string |
| http://purl.uniprot.org/citations/17964421 | http://purl.uniprot.org/core/volume | "43"xsd:string |
| http://purl.uniprot.org/citations/17964421 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17964421 |
| http://purl.uniprot.org/citations/17964421 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17964421 |
| http://purl.uniprot.org/uniprot/P22791#attribution-4DFE352241339261B123EA5CEB69D780 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17964421 |
| http://purl.uniprot.org/uniprot/#_P22791-mappedCitation-17964421 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17964421 |
| http://purl.uniprot.org/uniprot/P22791 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17964421 |