| http://purl.uniprot.org/citations/17970729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17970729 | http://www.w3.org/2000/01/rdf-schema#comment | "Olfactory sensory neurons respond to odorants increasing Ca(2+) concentrations in their chemosensory cilia. Calcium enters the cilia through cAMP-gated channels, activating Ca(2+)-dependent chloride or potassium channels. Calcium also has a fundamental role in odour adaptation, regulating cAMP turnover rate and the affinity of the cyclic nucleotide-gated channels for cAMP. It has been shown that a Na(+)/Ca(2+) exchanger (NCX) extrudes Ca(2+) from the cilia. Here we confirm previous evidence that olfactory cilia also express plasma membrane Ca(2+)-ATPase (PMCA), and show the first evidence supporting a role in Ca(2+) removal. Both transporters were detected by immunoblot of purified olfactory cilia membranes. The pump was also revealed by immunocytochemistry and immunohistochemistry. Inside-out cilia membrane vesicles transported Ca(2+) in an ATP-dependent fashion. PMCA activity was potentiated by luminal Ca(2+) (K(0.5) = 670 nm) and enhanced by calmodulin (CaM; K(0.5) = 31 nm). Both carboxyeosin (CE) and calmidazolium reduced Ca(2+) transport, as expected for a CaM-modulated PMCA. The relaxation time constant (tau) of the Ca(2+)-dependent Cl(-) current (272 +/-78 ms), indicative of luminal Ca(2+) decline, was increased by CE (2181 +/-437 ms), by omitting ATP (666 +/- 49 ms) and by raising pH (725 +/-65 ms), suggesting a role of the pump on Ca(2+) clearance. Replacement of external Na(+) by Li(+) had a similar effect (tau = 442 +/-8 ms), confirming the NCX involvement in Ca(2+) extrusion. The evidence suggests that both Ca(2+) transporters contribute to re-establish resting Ca(2+) levels in the cilia following olfactory responses."xsd:string |
| http://purl.uniprot.org/citations/17970729 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1460-9568.2007.05863.x"xsd:string |
| http://purl.uniprot.org/citations/17970729 | http://purl.uniprot.org/core/author | "Castillo K."xsd:string |
| http://purl.uniprot.org/citations/17970729 | http://purl.uniprot.org/core/author | "Delgado R."xsd:string |
| http://purl.uniprot.org/citations/17970729 | http://purl.uniprot.org/core/author | "Bacigalupo J."xsd:string |
| http://purl.uniprot.org/citations/17970729 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17970729 | http://purl.uniprot.org/core/name | "Eur J Neurosci"xsd:string |
| http://purl.uniprot.org/citations/17970729 | http://purl.uniprot.org/core/pages | "2524-2531"xsd:string |
| http://purl.uniprot.org/citations/17970729 | http://purl.uniprot.org/core/title | "Plasma membrane Ca(2+)-ATPase in the cilia of olfactory receptor neurons: possible role in Ca(2+) clearance."xsd:string |
| http://purl.uniprot.org/citations/17970729 | http://purl.uniprot.org/core/volume | "26"xsd:string |
| http://purl.uniprot.org/citations/17970729 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17970729 |
| http://purl.uniprot.org/citations/17970729 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17970729 |
| http://purl.uniprot.org/uniprot/#_A6IBU4-mappedCitation-17970729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17970729 |
| http://purl.uniprot.org/uniprot/#_P11506-mappedCitation-17970729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17970729 |
| http://purl.uniprot.org/uniprot/A6IBU4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17970729 |
| http://purl.uniprot.org/uniprot/P11506 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17970729 |