| http://purl.uniprot.org/citations/17973403 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17973403 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17973403 | http://www.w3.org/2000/01/rdf-schema#comment | "The function of the mlr6791 gene from Mesorhizobium loti MAFF303099 has been identified. This gene encodes 3-hydroxy-2-methylpyridine-4,5-dicarboxylate decarboxylase (HMPDdc), an enzyme involved in the catabolism of pyridoxal 5'-phosphate (Vitamin B6). This enzyme was overexpressed in Escherichia coli and characterized. HMPDdc is a 26 kDa protein that catalyzes the decarboxylation of 3-hydroxy-2-methylpyridine-4,5-dicarboxylate to 3-hydroxy-2-methylpyridine-5-carboxylate. The KM and kcat were found to be 366 microM and 0.6 s-1, respectively. The structure of this enzyme was determined at 1.9 A resolution using SAD phasing and belongs to the class II aldolase/adducin superfamily. While the decarboxylation of hydroxy-substituted benzene rings is a common motif in biosynthesis, the mechanism of this reaction is still poorly characterized. The structural studies described here suggest that catalysis of such decarboxylations proceeds by an aldolase-like mechanism."xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi701439j"xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi701439j"xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/author | "Begley T.P."xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/author | "Begley T.P."xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/author | "Ealick S.E."xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/author | "Ealick S.E."xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/author | "McCulloch K.M."xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/author | "McCulloch K.M."xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/author | "Mukherjee T."xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/author | "Mukherjee T."xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/pages | "13606-13615"xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/pages | "13606-13615"xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/title | "Gene identification and structural characterization of the pyridoxal 5'-phosphate degradative protein 3-hydroxy-2-methylpyridine-4,5-dicarboxylate decarboxylase from mesorhizobium loti MAFF303099."xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/title | "Gene identification and structural characterization of the pyridoxal 5'-phosphate degradative protein 3-hydroxy-2-methylpyridine-4,5-dicarboxylate decarboxylase from mesorhizobium loti MAFF303099."xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/volume | "46"xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://purl.uniprot.org/core/volume | "46"xsd:string |
| http://purl.uniprot.org/citations/17973403 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17973403 |
| http://purl.uniprot.org/citations/17973403 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17973403 |