| http://purl.uniprot.org/citations/17984224 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17984224 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17984224 | http://www.w3.org/2000/01/rdf-schema#comment | "Loading of export factors onto mRNAs is a key step in gene expression. In vertebrates, splicing plays a role in this process. Specific protein complexes, exon junction complex and transcription/export complex, are loaded onto mRNAs in a splicing-dependent manner, and adaptor proteins such as Aly/REF in the complexes in turn recruit mRNA exporter TAP-p15 onto the RNA. By contrast, how export factors are recruited onto intronless mRNAs is largely unknown. We previously showed that Aly/REF is preferentially associated with intronless mRNAs in the nucleus. Here we show that Aly/REF could preferentially bind intronless mRNAs in vitro and that this binding was stimulated by RNA helicase UAP56 in an ATP-dependent manner. Consistently, an ATP binding-deficient UAP56 mutant specifically inhibited mRNA export in Xenopus oocytes. Interestingly, ATP activated the RNA binding activity of UAP56 itself. ATP-bound UAP56 therefore bound to both RNA and Aly/REF, and as a result ATPase activity of UAP56 was cooperatively stimulated. These results are consistent with a model in which ATP-bound UAP56 chaperones Aly/REF onto RNA, ATP is then hydrolyzed, and UAP56 dissociates from RNA for the next round of Aly/REF recruitment. Our finding provides a mechanistic insight into how export factors are recruited onto mRNAs."xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.01341-07"xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.01341-07"xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/author | "Ohno M."xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/author | "Ohno M."xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/author | "Taniguchi I."xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/author | "Taniguchi I."xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/pages | "601-608"xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/pages | "601-608"xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/title | "ATP-dependent recruitment of export factor Aly/REF onto intronless mRNAs by RNA helicase UAP56."xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/title | "ATP-dependent recruitment of export factor Aly/REF onto intronless mRNAs by RNA helicase UAP56."xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/volume | "28"xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://purl.uniprot.org/core/volume | "28"xsd:string |
| http://purl.uniprot.org/citations/17984224 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17984224 |
| http://purl.uniprot.org/citations/17984224 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17984224 |
| http://purl.uniprot.org/citations/17984224 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17984224 |
| http://purl.uniprot.org/citations/17984224 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17984224 |
| http://purl.uniprot.org/uniprot/Q86V81 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/17984224 |
| http://purl.uniprot.org/uniprot/Q13838 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/17984224 |