| http://purl.uniprot.org/citations/17989281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17989281 | http://www.w3.org/2000/01/rdf-schema#comment | "The SM (Sec1/Munc18-like) protein Munc18-1 and the soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins syntaxin-1, SNAP-25, and synaptobrevin/VAMP (vesicle-associated membrane protein) constitute the core fusion machinery for synaptic vesicle exocytosis. Strikingly, Munc18-1 interacts with neuronal SNARE proteins in two distinct modes (i.e., with isolated syntaxin-1 alone in a "closed" conformation and with assembled SNARE complexes containing syntaxin-1 in an "open" conformation). However, it is unclear whether the two modes of Munc18/SNARE interactions are linked. We now show that both Munc18/SNARE interaction modes involve the same low-affinity binding of the extreme syntaxin-1 N terminus to Munc18-1, suggesting that this binding connects the two Munc18/SNARE interaction modes to each other. Using transfected cells as an in vitro assay system, we demonstrate that truncated syntaxins lacking a transmembrane region universally block exocytosis, but only if they contain a free intact N terminus. This block is enhanced by coexpression of either Munc18-1 or SNAP-25, suggesting that truncated syntaxins block exocytosis by forming an untethered inhibitory SNARE complex/Munc18-1 assembly in which the N-terminal syntaxin/Munc18 interaction is essential. Introduction of an N-terminal syntaxin peptide that disrupts this assembly blocks neurotransmitter release in the calyx of Held synapse, whereas a mutant peptide that does not disrupt the SNARE complex/Munc18 assembly has no effect. Viewed together, our data indicate that binding of Munc18 to the syntaxin N terminus unites different modes of Munc18/SNARE interactions and is essential for exocytic membrane fusion."xsd:string |
| http://purl.uniprot.org/citations/17989281 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.3655-07.2007"xsd:string |
| http://purl.uniprot.org/citations/17989281 | http://purl.uniprot.org/core/author | "Sun J."xsd:string |
| http://purl.uniprot.org/citations/17989281 | http://purl.uniprot.org/core/author | "Dai H."xsd:string |
| http://purl.uniprot.org/citations/17989281 | http://purl.uniprot.org/core/author | "Sudhof T.C."xsd:string |
| http://purl.uniprot.org/citations/17989281 | http://purl.uniprot.org/core/author | "Khvotchev M."xsd:string |
| http://purl.uniprot.org/citations/17989281 | http://purl.uniprot.org/core/author | "Dulubova I."xsd:string |
| http://purl.uniprot.org/citations/17989281 | http://purl.uniprot.org/core/author | "Rizo J."xsd:string |
| http://purl.uniprot.org/citations/17989281 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17989281 | http://purl.uniprot.org/core/name | "J Neurosci"xsd:string |
| http://purl.uniprot.org/citations/17989281 | http://purl.uniprot.org/core/pages | "12147-12155"xsd:string |
| http://purl.uniprot.org/citations/17989281 | http://purl.uniprot.org/core/title | "Dual modes of Munc18-1/SNARE interactions are coupled by functionally critical binding to syntaxin-1 N terminus."xsd:string |
| http://purl.uniprot.org/citations/17989281 | http://purl.uniprot.org/core/volume | "27"xsd:string |
| http://purl.uniprot.org/citations/17989281 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17989281 |
| http://purl.uniprot.org/citations/17989281 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17989281 |
| http://purl.uniprot.org/uniprot/#_P61764-mappedCitation-17989281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17989281 |
| http://purl.uniprot.org/uniprot/#_P63027-mappedCitation-17989281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17989281 |
| http://purl.uniprot.org/uniprot/#_O14795-mappedCitation-17989281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17989281 |
| http://purl.uniprot.org/uniprot/#_O14810-mappedCitation-17989281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17989281 |
| http://purl.uniprot.org/uniprot/#_P21579-mappedCitation-17989281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17989281 |
| http://purl.uniprot.org/uniprot/#_Q13136-mappedCitation-17989281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17989281 |
| http://purl.uniprot.org/uniprot/#_P20336-mappedCitation-17989281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17989281 |
| http://purl.uniprot.org/uniprot/#_Q16623-mappedCitation-17989281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17989281 |
| http://purl.uniprot.org/uniprot/#_Q16572-mappedCitation-17989281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17989281 |