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http://purl.uniprot.org/citations/17991872http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17991872http://www.w3.org/2000/01/rdf-schema#comment"

Background

We have previously reported the potentiation of PDGF-BB-induced chemotaxis of fibroblasts, vascular smooth muscle cells, and endothelial cells by FVIIa. Here we studied the role of TF/FVIIa and the induced signaling pathways in regulation of chemotaxis of human monocytes, fibroblasts, and porcine aorta endothelial cells.

Methods and results

Human monocytes were obtained by using Ficoll-Paque gradient and the MACS system (for highly purified population), fibroblasts and PAE cells have been characterized previously. Inhibitors of selected signaling intermediates were used, and the effect of TF/FVIIa on the migratory response of all cells to chemotactic agents was analyzed. The induced signaling was studied by immunoprecipitation and Western blotting. TF/FVIIa complex selectively enhanced PDGF-BB-induced chemotaxis in a Src-family, PLC, and PAR-2-dependent manner. Using PAE cells we identified c-Src and c-Yes as the Src-family members activated by TF/FVIIa. We report for the first time the PAR-2 and Src family-dependent transactivation of PDGFRbeta by TF/FVIIa involving phosphorylation of a subset of PDGFRbeta tyrosines.

Conclusions

The described transactivation is a likely mechanism of TF/FVIIa-mediated regulation of PDGF-BB-induced chemotaxis. Similar behavior of 3 principally different cell types in our experimental setup may reflect a general function of TF in regulation of cell migration."xsd:string
http://purl.uniprot.org/citations/17991872http://purl.org/dc/terms/identifier"doi:10.1161/atvbaha.107.155754"xsd:string
http://purl.uniprot.org/citations/17991872http://purl.uniprot.org/core/author"Siegbahn A."xsd:string
http://purl.uniprot.org/citations/17991872http://purl.uniprot.org/core/author"Velling T."xsd:string
http://purl.uniprot.org/citations/17991872http://purl.uniprot.org/core/author"Nordin A."xsd:string
http://purl.uniprot.org/citations/17991872http://purl.uniprot.org/core/author"Johnell M."xsd:string
http://purl.uniprot.org/citations/17991872http://purl.uniprot.org/core/author"Aberg M."xsd:string
http://purl.uniprot.org/citations/17991872http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/17991872http://purl.uniprot.org/core/name"Arterioscler Thromb Vasc Biol"xsd:string
http://purl.uniprot.org/citations/17991872http://purl.uniprot.org/core/pages"135-141"xsd:string
http://purl.uniprot.org/citations/17991872http://purl.uniprot.org/core/title"TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC."xsd:string
http://purl.uniprot.org/citations/17991872http://purl.uniprot.org/core/volume"28"xsd:string
http://purl.uniprot.org/citations/17991872http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/17991872
http://purl.uniprot.org/citations/17991872http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/17991872
http://purl.uniprot.org/uniprot/P08709#attribution-8103F3BA5EF05136F8AFE38069077698http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/17991872
http://purl.uniprot.org/uniprot/P01127#attribution-8103F3BA5EF05136F8AFE38069077698http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/17991872
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http://purl.uniprot.org/uniprot/P13726#attribution-6ACB83E1E3B16D29CE724543AA72C917http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/17991872
http://purl.uniprot.org/uniprot/P13726#attribution-8103F3BA5EF05136F8AFE38069077698http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/17991872
http://purl.uniprot.org/uniprot/#_A3RKG7-mappedCitation-17991872http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17991872
http://purl.uniprot.org/uniprot/#_A0A1G4P1I6-mappedCitation-17991872http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17991872
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http://purl.uniprot.org/uniprot/#_A3RMR1-mappedCitation-17991872http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17991872
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