| http://purl.uniprot.org/citations/17996400 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17996400 | http://www.w3.org/2000/01/rdf-schema#comment | "Systematically annotating function of enzymes that belong to large protein families encoded in a single eukaryotic genome is a very challenging task. We carried out such an exercise to annotate function for serine-protease family of the trypsin fold in Drosophila melanogaster, with an emphasis on annotating serine-protease homologues (SPHs) that may have lost their catalytic function. Our approach involves data mining and data integration to provide function annotations for 190 Drosophila gene products containing serine-protease-like domains, of which 35 are SPHs. This was accomplished by analysis of structure-function relationships, gene-expression profiles, large-scale protein-protein interaction data, literature mining and bioinformatic tools. We introduce functional residue clustering (FRC), a method that performs hierarchical clustering of sequences using properties of functionally important residues and utilizes correlation co-efficient as a quantitative similarity measure to transfer in vivo substrate specificities to proteases. We show that the efficiency of transfer of substrate-specificity information using this method is generally high. FRC was also applied on Drosophila proteases to assign putative competitive inhibitor relationships (CIRs). Microarray gene-expression data were utilized to uncover a large-scale and dual involvement of proteases in development and in immune response. We found specific recruitment of SPHs and proteases with CLIP domains in immune response, suggesting evolution of a new function for SPHs. We also suggest existence of separate downstream protease cascades for immune response against bacterial/fungal infections and parasite/parasitoid infections. We verify quality of our annotations using information from RNAi screens and other evidence types. Utilization of such multi-fold approaches results in 10-fold increase of function annotation for Drosophila serine proteases and demonstrates value in increasing annotations in multiple genomes."xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.gene.2007.10.012"xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/author | "Bork P."xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/author | "Jensen L.J."xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/author | "Rodrigues V."xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/author | "Mason C."xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/author | "Shah P.K."xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/author | "White K.P."xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/author | "Sowdhamini R."xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/author | "Tripathi L.P."xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/author | "Furlong E.E."xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/author | "Gahnim M."xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/pages | "199-215"xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/title | "Enhanced function annotations for Drosophila serine proteases: a case study for systematic annotation of multi-member gene families."xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://purl.uniprot.org/core/volume | "407"xsd:string |
| http://purl.uniprot.org/citations/17996400 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17996400 |
| http://purl.uniprot.org/citations/17996400 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17996400 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KEK5-mappedCitation-17996400 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17996400 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KFB1-mappedCitation-17996400 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17996400 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KI65-mappedCitation-17996400 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17996400 |
| http://purl.uniprot.org/uniprot/#_A0A0B4LEW8-mappedCitation-17996400 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17996400 |
| http://purl.uniprot.org/uniprot/#_E1JGR0-mappedCitation-17996400 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17996400 |