| http://purl.uniprot.org/citations/17997327 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17997327 | http://www.w3.org/2000/01/rdf-schema#comment | "We report here the first biochemical and structural characterization of the respiratory syncytial virus (RSV) NS1 protein. We have used a pET-ubiquitin expression system to produce respiratory syncytial virus (RSV) NS1 protein in E. coli that contains a hexahistidine-tag on either the amino- or carboxyl-terminus (His(6)-NS1 and NS1-His(6), respectively). We have been able to isolate milligram quantities of highly purified His(6)-NS1 and NS1-His(6) by nickel affinity chromatography. Generation of recombinant RSV indicated that addition of the hexahistidine tag to the C-terminus of NS1 slightly decreased viral replication competence whereas addition of the tag to the N-terminus had no observable effect. Therefore, we performed a comprehensive biochemical and biophysical characterization on His(6)-NS1. His(6)-NS1 is monodisperse in solution as determined by dynamic light scattering analysis. Both gel filtration and analytical ultracentrifugation showed that His(6)-NS1 is predominantly a monomer. In agreement with theoretical predictions, circular dichroism spectroscopy showed that His(6)-NS1 contains 21% alpha-helices, 34% beta-sheets, and 45% undefined structure. Immunization with purified His(6)-NS1 generated an antiserum that specifically recognizes NS1 by immunoprecipitation from HEp-2 cells infected by RSV, indicating that His(6)-NS1 resembles native NS1. The availability of purified RSV NS1 will permit biochemical and structural investigations providing insight into the function of NS1 in viral replication and interferon antagonism."xsd:string |
| http://purl.uniprot.org/citations/17997327 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.pep.2007.09.017"xsd:string |
| http://purl.uniprot.org/citations/17997327 | http://purl.uniprot.org/core/author | "Ling Z."xsd:string |
| http://purl.uniprot.org/citations/17997327 | http://purl.uniprot.org/core/author | "Teng M.N."xsd:string |
| http://purl.uniprot.org/citations/17997327 | http://purl.uniprot.org/core/author | "Tran K.C."xsd:string |
| http://purl.uniprot.org/citations/17997327 | http://purl.uniprot.org/core/author | "Arnold J.J."xsd:string |
| http://purl.uniprot.org/citations/17997327 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/17997327 | http://purl.uniprot.org/core/name | "Protein Expr Purif"xsd:string |
| http://purl.uniprot.org/citations/17997327 | http://purl.uniprot.org/core/pages | "261-270"xsd:string |
| http://purl.uniprot.org/citations/17997327 | http://purl.uniprot.org/core/title | "Purification and characterization of recombinant human respiratory syncytial virus nonstructural protein NS1."xsd:string |
| http://purl.uniprot.org/citations/17997327 | http://purl.uniprot.org/core/volume | "57"xsd:string |
| http://purl.uniprot.org/citations/17997327 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17997327 |
| http://purl.uniprot.org/citations/17997327 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17997327 |
| http://purl.uniprot.org/uniprot/#_P0DOE9-mappedCitation-17997327 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17997327 |
| http://purl.uniprot.org/uniprot/P0DOE9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17997327 |