http://purl.uniprot.org/citations/18023351 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/18023351 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundmiRNAs are an abundant class of small, endogenous regulatory RNAs. Although it is now appreciated that miRNAs are involved in a broad range of biological processes, relatively little is known about the actual mechanism by which miRNAs downregulate target gene expression. An exploration of which protein cofactors are necessary for a miRNA to downregulate a target gene should reveal more fully the molecular mechanisms by which miRNAs are processed, trafficked, and regulate their target genes.ResultsA weak allele of the C. elegans miRNA gene let-7 was used as a sensitized genetic background for a whole-genome RNAi screen to detect miRNA pathway genes, and 213 candidate miRNA pathway genes were identified. About 2/3 of the 61 candidates with the strongest phenotype were validated through genetic tests examining the dependence of the let-7 phenotype on target genes known to function in the let-7 pathway. Biochemical tests for let-7 miRNA production place the function of nearly all of these new miRNA pathway genes downstream of let-7 expression and processing. By monitoring the downregulation of the protein product of the lin-14 mRNA, which is the target of the lin-4 miRNA, we have identified 19 general miRNA pathway genes.ConclusionsThe 213 candidate miRNA pathway genes identified could act at steps that produce and traffic miRNAs or in downstream steps that detect miRNA::mRNA duplexes to regulate mRNA translation. The 19 validated general miRNA pathway genes are good candidates for genes that may define protein cofactors for sorting or targeting miRNA::mRNA duplexes, or for recognizing the miRNA base-paired to the target mRNA to downregulate translation."xsd:string |
http://purl.uniprot.org/citations/18023351 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cub.2007.10.058"xsd:string |
http://purl.uniprot.org/citations/18023351 | http://purl.uniprot.org/core/author | "Xu J."xsd:string |
http://purl.uniprot.org/citations/18023351 | http://purl.uniprot.org/core/author | "Ruvkun G."xsd:string |
http://purl.uniprot.org/citations/18023351 | http://purl.uniprot.org/core/author | "Parry D.H."xsd:string |
http://purl.uniprot.org/citations/18023351 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
http://purl.uniprot.org/citations/18023351 | http://purl.uniprot.org/core/name | "Curr Biol"xsd:string |
http://purl.uniprot.org/citations/18023351 | http://purl.uniprot.org/core/pages | "2013-2022"xsd:string |
http://purl.uniprot.org/citations/18023351 | http://purl.uniprot.org/core/title | "A whole-genome RNAi Screen for C. elegans miRNA pathway genes."xsd:string |
http://purl.uniprot.org/citations/18023351 | http://purl.uniprot.org/core/volume | "17"xsd:string |
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