| http://purl.uniprot.org/citations/18029012 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18029012 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18029012 | http://www.w3.org/2000/01/rdf-schema#comment | "A previous study has demonstrated that the ubiquitous plasma membrane Ca(2+) pump PMCA4 interacted with isoform epsilon of the 14-3-3 protein, whereas the nervous tissue-specific PMCA2 did not. The 14-3-3 proteins are widely expressed small acidic proteins, which modulate cell signaling, intracellular trafficking, transcription and apoptosis. The investigation has been extended to the other tissue-restricted pump (PMCA3) and to the other ubiquitous pump (PMCA1). At variance with PMCA2, PMCA3 interacted with the 14-3-3epsilon protein in a two-hybrid system assay, which could not be used for PMCA1. The 14-3-3epsilon protein immunoprecipitated with both PMCA3 and PMCA1 when expressed in HeLa cells. Pull-down experiments using GST-PMCA1 and GST-PMCA3 fusion products confirmed the interaction of both pumps with the 14-3-3epsilon protein. The binding was phosphorylation-independent with both PMCA3 and PMCA1. The 14-3-3zeta isoform also interacted with PMCA3; however, it did not interact with PMCA1. The effect of the interaction on the activity of the two pumps, and thus on the homeostasis of Ca(2+), was investigated by co-expressing the 14-3-3epsilon protein and PMCA3 or PMCA1 in CHO cells together with the recombinant Ca(2+) indicator aequorin: the ability of cells to re-establish the basal Ca(2+) concentration following a Ca(2+) transient induced by an InsP(3)-producing agonist was substantially decreased with both pumps, indicating that the interaction with the 14-3-3 protein inhibited the activity of both PMCA3 and PMCA1."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ceca.2007.09.003"xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ceca.2007.09.003"xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/author | "Brini M."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/author | "Brini M."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/author | "Carafoli E."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/author | "Carafoli E."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/author | "Di Leva F."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/author | "Di Leva F."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/author | "Domi T."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/author | "Domi T."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/author | "Linde C.I."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/author | "Linde C.I."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/author | "Tosatto S.C."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/author | "Tosatto S.C."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/name | "Cell Calcium"xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/name | "Cell Calcium"xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/pages | "550-561"xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/pages | "550-561"xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/title | "Inhibitory interaction of the 14-3-3 proteins with ubiquitous (PMCA1) and tissue-specific (PMCA3) isoforms of the plasma membrane Ca2+ pump."xsd:string |
| http://purl.uniprot.org/citations/18029012 | http://purl.uniprot.org/core/title | "Inhibitory interaction of the 14-3-3 proteins with ubiquitous (PMCA1) and tissue-specific (PMCA3) isoforms of the plasma membrane Ca2+ pump."xsd:string |