| http://purl.uniprot.org/citations/18032517 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18032517 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18032517 | http://www.w3.org/2000/01/rdf-schema#comment | "A key function of the Nef protein of immunodeficiency viruses is the downregulation of the T-cell and macrophage coreceptor, CD4, from the surfaces of infected cells. CD4 downregulation depends on a conserved (D/E)XXXL(L/I)-type dileucine motif in the C-terminal, flexible loop of Nef, which mediates binding to the clathrin adaptor complexes AP-1, AP-2, and AP-3. We now report the identification of a consensus (D/E)D motif within this loop as a second, conserved determinant of interaction of Nef with AP-2, though not with AP-1 and AP-3. Mutations in this diacidic motif abrogate both AP-2 binding and CD4 downregulation. We also show that a dileucine motif from tyrosinase, both in its native context and in the context of Nef, can bind to AP-2 independently of a diacidic motif. These results thus identify a novel type of AP-2 interaction determinant, support the notion that AP-2 is the key clathrin adaptor for the downregulation of CD4 by Nef, and reveal a previously unrecognized diversity among dileucine sorting signals."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.01874-07"xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.01874-07"xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/author | "Chaudhuri R."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/author | "Chaudhuri R."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/author | "Yang P."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/author | "Yang P."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/author | "Hurley J.H."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/author | "Hurley J.H."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/author | "Bonifacino J.S."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/author | "Bonifacino J.S."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/author | "Smith W.J."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/author | "Smith W.J."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/author | "Lindwasser O.W."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/author | "Lindwasser O.W."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/name | "J. Virol."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/name | "J. Virol."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/pages | "1166-1174"xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/pages | "1166-1174"xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/title | "A diacidic motif in human immunodeficiency virus type 1 Nef is a novel determinant of binding to AP-2."xsd:string |
| http://purl.uniprot.org/citations/18032517 | http://purl.uniprot.org/core/title | "A diacidic motif in human immunodeficiency virus type 1 Nef is a novel determinant of binding to AP-2."xsd:string |