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http://purl.uniprot.org/citations/18036244http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18036244http://www.w3.org/2000/01/rdf-schema#comment"

Background

Several subtypes of HIV-1 circulate in infected people worldwide, including subtype B in the United States and subtype C in Africa and India. To understand the biological properties of HIV-1 subtype C, including cellular tropism, virus entry, replication efficiency and cytopathic effects, we reciprocally inserted our previously characterized envelope V3-V5 regions derived from 9 subtype C infected patients from India into a subtype B molecular clone, pNL4-3. Equal amounts of the chimeric viruses were used to infect T-lymphocyte cell lines (A3.01 and MT-2), coreceptor cell lines (U373-MAGI-CCR5/CXCR4), primary blood T-lymphocytes (PBL) and monocyte-derived macrophages (MDM).

Results

We found that subtype C envelope V3-V5 region chimeras failed to replicate in T-lymphocyte cell lines but replicated in PBL and MDM. In addition, these chimeras were able to infect U373MAGI-CD4+-CCR5+ but not U373MAGI-CD4+-CXCR4+ cell line, suggesting CCR5 coreceptor utilization and R5 phenotypes. These subtype C chimeras were unable to induce syncytia in MT-2 cells, indicative of non-syncytium inducing (NSI) phenotypes. More importantly, the subtype C envelope chimeras replicated at higher levels in PBL and MDM compared with subtype B chimeras and isolates. Furthermore, the higher levels subtype C chimeras replication in PBL and MDM correlated with increased virus entry in U373MAGI-CD4+-CCR5+.

Conclusion

Taken together, these results suggest that the envelope V3 to V5 regions of subtype C contributed to higher levels of HIV-1 replication compared with subtype B chimeras, which may contribute to higher viral loads and faster disease progression in subtype C infected individuals than other subtypes as well as rapid HIV-1 subtype C spread in India."xsd:string
http://purl.uniprot.org/citations/18036244http://purl.org/dc/terms/identifier"doi:10.1186/1743-422x-4-126"xsd:string
http://purl.uniprot.org/citations/18036244http://purl.uniprot.org/core/author"Jameel S."xsd:string
http://purl.uniprot.org/citations/18036244http://purl.uniprot.org/core/author"Das S.R."xsd:string
http://purl.uniprot.org/citations/18036244http://purl.uniprot.org/core/author"Ahmad N."xsd:string
http://purl.uniprot.org/citations/18036244http://purl.uniprot.org/core/author"Ramakrishnan R."xsd:string
http://purl.uniprot.org/citations/18036244http://purl.uniprot.org/core/author"Sehgal S."xsd:string
http://purl.uniprot.org/citations/18036244http://purl.uniprot.org/core/author"Gopalan S."xsd:string
http://purl.uniprot.org/citations/18036244http://purl.uniprot.org/core/author"Sundaravaradan V."xsd:string
http://purl.uniprot.org/citations/18036244http://purl.uniprot.org/core/date"2007"xsd:gYear
http://purl.uniprot.org/citations/18036244http://purl.uniprot.org/core/name"Virol J"xsd:string
http://purl.uniprot.org/citations/18036244http://purl.uniprot.org/core/pages"126"xsd:string
http://purl.uniprot.org/citations/18036244http://purl.uniprot.org/core/title"Role of HIV-1 subtype C envelope V3 to V5 regions in viral entry, coreceptor utilization and replication efficiency in primary T-lymphocytes and monocyte-derived macrophages."xsd:string
http://purl.uniprot.org/citations/18036244http://purl.uniprot.org/core/volume"4"xsd:string
http://purl.uniprot.org/citations/18036244http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18036244
http://purl.uniprot.org/citations/18036244http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/18036244
http://purl.uniprot.org/uniprot/#_A0A089G6S9-mappedCitation-18036244http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18036244
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http://purl.uniprot.org/uniprot/#_A0A089G7G0-mappedCitation-18036244http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18036244
http://purl.uniprot.org/uniprot/#_A0A089G7G7-mappedCitation-18036244http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18036244
http://purl.uniprot.org/uniprot/#_A0A089G7G9-mappedCitation-18036244http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18036244
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http://purl.uniprot.org/uniprot/#_A0A089G3N4-mappedCitation-18036244http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18036244
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