| http://purl.uniprot.org/citations/18044992 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18044992 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18044992 | http://www.w3.org/2000/01/rdf-schema#comment | "Vaults are the largest known cytoplasmic ribonucleoprotein structures and may function in innate immunity. The vault shell self-assembles from 96 copies of major vault protein and encapsulates two other proteins and a small RNA. We crystallized rat liver vaults and several recombinant vaults, all among the largest non-icosahedral particles to have been crystallized. The best crystals thus far were formed from empty vaults built from a cysteine-tag construct of major vault protein (termed cpMVP vaults), diffracting to about 9-A resolution. The asymmetric unit contains a half vault of molecular mass 4.65 MDa. X-ray phasing was initiated by molecular replacement, using density from cryo-electron microscopy (cryo-EM). Phases were improved by density modification, including concentric 24- and 48-fold rotational symmetry averaging. From this, the continuous cryo-EM electron density separated into domain-like blocks. A draft atomic model of cpMVP was fit to this improved density from 15 domain models. Three domains were adapted from a nuclear magnetic resonance substructure. Nine domain models originated in ab initio tertiary structure prediction. Three C-terminal domains were built by fitting poly-alanine to the electron density. Locations of loops in this model provide sites to test vault functions and to exploit vaults as nanocapsules."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pbio.0050318"xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pbio.0050318"xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/author | "Anderson D.H."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/author | "Anderson D.H."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/author | "Eisenberg D."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/author | "Eisenberg D."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/author | "Rome L.H."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/author | "Rome L.H."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/author | "Sievers S.A."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/author | "Sievers S.A."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/author | "Kickhoefer V.A."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/author | "Kickhoefer V.A."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/name | "PLoS Biol."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/name | "PLoS Biol."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/pages | "E318"xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/pages | "E318"xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/title | "Draft crystal structure of the vault shell at 9-A resolution."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/title | "Draft crystal structure of the vault shell at 9-A resolution."xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/volume | "5"xsd:string |
| http://purl.uniprot.org/citations/18044992 | http://purl.uniprot.org/core/volume | "5"xsd:string |