| http://purl.uniprot.org/citations/18056989 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18056989 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18056989 | http://www.w3.org/2000/01/rdf-schema#comment | "We previously showed that the 44-kDa serine/threonine kinase Pim-1 (Pim-1L) can protect prostate cancer cells from apoptosis induced by chemotherapeutic drugs (Xie, Y., Xu, K., Dai, B., Guo, Z., Jiang, T., Chen, H., and Qiu, Y. (2006) Oncogene 25, 70-78). To further explore the mechanisms of Pim-1L-mediated resistance to chemotherapeutic drugs in prostate cancer cells, we employed a yeast two-hybrid screening to identify cellular proteins that were associated with Pim-1L, and we found the ABC transporter BCRP/ABCG2 as one of the potential interacting partners of Pim-1L. We also showed that the expression level of Pim-1L and BCRP was up-regulated in mitoxantrone and docetaxel-resistant prostate cancer cell lines. Pim-1L was co-localized with BCRP on the plasma membrane and induced phosphorylation of BCRP at threonine 362. Knocking-down Pim-1L expression in the drug-resistant prostate cancer cells abolished multimer formation of endogenous BCRP and resensitized the resistant cells to chemotherapeutic drugs suggesting that BCRP phosphorylation induced by Pim-1L was essential for its functionality. This is further corroborated by our finding that the plasma membrane localization and drug-resistant activity of BCRP were compromised by T362A mutation. Our data suggest that Pim-1L may protect prostate cancer cells from apoptosis, at least in part, through regulation of transmembrane drug efflux pump. These findings may provide a potential therapeutic approach by disrupting Pim-1 signaling to reverse BCRP-mediated multidrug resistance."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m707773200"xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m707773200"xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Chen H."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Chen H."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Guo Z."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Guo Z."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Qiu Y."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Qiu Y."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Xie Y."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Xie Y."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Yang X."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Yang X."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Xu K."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Xu K."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Nakanishi T."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Nakanishi T."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Fazli L."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Fazli L."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Gleave M.E."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Gleave M.E."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Linn D.E."xsd:string |
| http://purl.uniprot.org/citations/18056989 | http://purl.uniprot.org/core/author | "Linn D.E."xsd:string |