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http://purl.uniprot.org/citations/1806555http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/1806555http://www.w3.org/2000/01/rdf-schema#comment"An epoxy-activated silica column (50 cm x 0.45 cm I.D.) was derivatized with 8-[6-aminohexyl)amino]-2'-phosphoadenosine-5'-diphosphoribose; the bound ligand concentration was 11.4 mumol/g of dry silica, and the useful loading capacity was 2.3 mg of glutathione reductase. The new high-performance liquid chromatographic column specifically retained NADP(+)-dependent enzymes, which were quantitatively eluted specifically by NADP+ or, with better resolution, by potassium chloride. The new high-performance liquid chromatographic support was applied to the purification of glutathione reductase and glucose-6-phosphate dehydrogenase from cell-free extracts of baker's yeast, fish liver and rabbit hemolysates, with high recoveries and excellent purification factors."xsd:string
http://purl.uniprot.org/citations/1806555http://purl.org/dc/terms/identifier"doi:10.1016/0021-9673(91)80024-b"xsd:string
http://purl.uniprot.org/citations/1806555http://purl.uniprot.org/core/author"Lopez-Barea J."xsd:string
http://purl.uniprot.org/citations/1806555http://purl.uniprot.org/core/author"Alhama J."xsd:string
http://purl.uniprot.org/citations/1806555http://purl.uniprot.org/core/author"Toribio F."xsd:string
http://purl.uniprot.org/citations/1806555http://purl.uniprot.org/core/date"1991"xsd:gYear
http://purl.uniprot.org/citations/1806555http://purl.uniprot.org/core/name"J Chromatogr"xsd:string
http://purl.uniprot.org/citations/1806555http://purl.uniprot.org/core/pages"51-59"xsd:string
http://purl.uniprot.org/citations/1806555http://purl.uniprot.org/core/title"High-performance affinity chromatography of NADP+ dehydrogenases from cell-free extracts using a nucleotide analogue as general ligand."xsd:string
http://purl.uniprot.org/citations/1806555http://purl.uniprot.org/core/volume"586"xsd:string
http://purl.uniprot.org/citations/1806555http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/1806555
http://purl.uniprot.org/citations/1806555http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/1806555
http://purl.uniprot.org/uniprot/#_P41921-mappedCitation-1806555http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/1806555
http://purl.uniprot.org/uniprot/#_P11412-mappedCitation-1806555http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/1806555
http://purl.uniprot.org/uniprot/P11412http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/1806555
http://purl.uniprot.org/uniprot/P41921http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/1806555