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http://purl.uniprot.org/citations/18070436http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18070436http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To examine the expression and subcellular localization of transcription factor USF1 in odontoblasts and investigate whether nuclear translocation occurs under stimuli.

Methods

Odontoblasts MDPC-23 were cultured on coverslips and divided into 2 groups. Group 1 received no stimuli, and group 2 was stimulated by nicotine with various concentrations respectively for 1h. Then the mountings of odontoblasts were prepared and immunocytochemical staining was performed with specific USF1 antibody via SABC method. Hela cells were used as positive control.

Results

The staining was positive in the cytoplasm of odontoblasts in group 1, but in the nuclei of Hela cells and in 100 mg/L nicotine-stimulated odontoblasts in group 2.

Conclusions

There exists USF1 protein in odontoblasts, which locates in the cytoplasm and could translocate into nuclei under the stimulation of nicotine."xsd:string
http://purl.uniprot.org/citations/18070436http://purl.uniprot.org/core/author"Fang J."xsd:string
http://purl.uniprot.org/citations/18070436http://purl.uniprot.org/core/author"Wen L.Y."xsd:string
http://purl.uniprot.org/citations/18070436http://purl.uniprot.org/core/author"Wang X.J."xsd:string
http://purl.uniprot.org/citations/18070436http://purl.uniprot.org/core/author"Wu L.A."xsd:string
http://purl.uniprot.org/citations/18070436http://purl.uniprot.org/core/author"Yang F.S."xsd:string
http://purl.uniprot.org/citations/18070436http://purl.uniprot.org/core/date"2007"xsd:gYear
http://purl.uniprot.org/citations/18070436http://purl.uniprot.org/core/name"Zhonghua Kou Qiang Yi Xue Za Zhi"xsd:string
http://purl.uniprot.org/citations/18070436http://purl.uniprot.org/core/pages"559-560"xsd:string
http://purl.uniprot.org/citations/18070436http://purl.uniprot.org/core/title"[Expression, subcellular localization and nuclear translocation of transcription factor up stream stimulatory factor-1 in odontoblasts]."xsd:string
http://purl.uniprot.org/citations/18070436http://purl.uniprot.org/core/volume"42"xsd:string
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