| http://purl.uniprot.org/citations/18070921 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18070921 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18070921 | http://www.w3.org/2000/01/rdf-schema#comment | "The transporter Ccc1 imports iron into the vacuole, which is the major site of iron storage in fungi and plants. CCC1 mRNA is destabilized under low-iron conditions by the binding of Cth1 and Cth2 to the 3' untranslated region (S. Puig, E. Askeland, and D. J. Thiele, Cell 120:99-110, 2005). Here, we show that the transcription of CCC1 is stimulated by iron through a Yap consensus site in the CCC1 promoter. We identified YAP5 as being the iron-sensitive transcription factor and show that a yap5Delta strain is sensitive to high iron. Green fluorescent protein-tagged Yap5 is localized to the nucleus and occupies the CCC1 promoter independent of the iron concentration. Yap5 contains two cysteine-rich domains, and the mutation of the cysteines to alanines in each of the domains affects the transcription of CCC1 but not DNA binding. The fusion of the Yap5 cysteine-containing domains to a GAL4 DNA binding domain results in iron-sensitive GAL1-lacZ expression. Iron affects the sulfhydryl status of Yap5, which is indicative of the generation of intramolecular disulfide bonds. These results show that Yap5 is an iron-sensing transcription factor and that iron regulates transcriptional activation."xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.01219-07"xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.01219-07"xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/author | "Li L."xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/author | "Li L."xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/author | "Ward D.M."xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/author | "Ward D.M."xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/author | "Kaplan J."xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/author | "Kaplan J."xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/author | "Bagley D."xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/author | "Bagley D."xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/pages | "1326-1337"xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/pages | "1326-1337"xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/title | "Yap5 is an iron-responsive transcriptional activator that regulates vacuolar iron storage in yeast."xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/title | "Yap5 is an iron-responsive transcriptional activator that regulates vacuolar iron storage in yeast."xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/volume | "28"xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://purl.uniprot.org/core/volume | "28"xsd:string |
| http://purl.uniprot.org/citations/18070921 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18070921 |
| http://purl.uniprot.org/citations/18070921 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18070921 |