| http://purl.uniprot.org/citations/18089563 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18089563 | http://www.w3.org/2000/01/rdf-schema#comment | "Epithelial cell-cell interactions require localized adhesive interactions between E-cadherin on opposing membranes and the activation of downstream signaling pathways that affect membrane and actin dynamics. However, it is not known whether E-cadherin engagement and activation of these signaling pathways are locally coordinated or whether signaling is sustained or locally down-regulated like other receptor-mediated pathways. To obtain high spatiotemporal resolution of immediate-early signaling events upon E-cadherin engagement, we used laser tweezers to place beads coated with functional E-cadherin extracellular domain on cells. We show that cellular E-cadherin accumulated rapidly around beads, reaching a sustained plateau level in 1-3 min. Phosphoinositides and Rac1 co-accumulated with E-cadherin, reached peak levels with E-cadherin, but then rapidly dispersed. Both E-cadherin and Rac1 accumulated independently of Rac1 GTP binding/hydrolysis, but these activities were required for Rac1 dispersal. E-cadherin accumulation was dependent on membrane dynamics and actin polymerization, but actin did not stably co-accumulate with E-cadherin; mathematical modeling showed that diffusion-mediated trapping could account for the initial E-cadherin accumulation. We propose that initial E-cadherin accumulation requires active membrane dynamics and involves diffusion-mediated trapping at contact sites; to propagate further contacts, phosphatidylinositol 3-kinase and Rac1 are transiently activated by E-cadherin engagement and initiate a new round of membrane dynamics, but they are subsequently suppressed at that site to allow maintenance of weak E-cadherin mediated adhesion."xsd:string |
| http://purl.uniprot.org/citations/18089563 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m705209200"xsd:string |
| http://purl.uniprot.org/citations/18089563 | http://purl.uniprot.org/core/author | "Nelson W.J."xsd:string |
| http://purl.uniprot.org/citations/18089563 | http://purl.uniprot.org/core/author | "Perez T.D."xsd:string |
| http://purl.uniprot.org/citations/18089563 | http://purl.uniprot.org/core/author | "Sheetz M.P."xsd:string |
| http://purl.uniprot.org/citations/18089563 | http://purl.uniprot.org/core/author | "Tamada M."xsd:string |
| http://purl.uniprot.org/citations/18089563 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18089563 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/18089563 | http://purl.uniprot.org/core/pages | "5014-5022"xsd:string |
| http://purl.uniprot.org/citations/18089563 | http://purl.uniprot.org/core/title | "Immediate-early signaling induced by E-cadherin engagement and adhesion."xsd:string |
| http://purl.uniprot.org/citations/18089563 | http://purl.uniprot.org/core/volume | "283"xsd:string |
| http://purl.uniprot.org/citations/18089563 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18089563 |
| http://purl.uniprot.org/citations/18089563 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18089563 |
| http://purl.uniprot.org/uniprot/#_F1PAA9-mappedCitation-18089563 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18089563 |
| http://purl.uniprot.org/uniprot/#_Q95LE0-mappedCitation-18089563 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18089563 |
| http://purl.uniprot.org/uniprot/F1PAA9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18089563 |
| http://purl.uniprot.org/uniprot/Q95LE0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18089563 |