| http://purl.uniprot.org/citations/18094046 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18094046 | http://www.w3.org/2000/01/rdf-schema#comment | "Cholera toxin (CT) intoxicates cells by using its receptor-binding B subunit (CTB) to traffic from the plasma membrane to the endoplasmic reticulum (ER). In this compartment, the catalytic A1 subunit (CTA1) is unfolded by protein disulfide isomerase (PDI) and retro-translocated to the cytosol where it triggers a signaling cascade, leading to secretory diarrhea. How CT is targeted to the site of retro-translocation in the ER membrane to initiate translocation is unclear. Using a semipermeabilized-cell retro-translocation assay, we demonstrate that a dominant-negative Derlin-1-YFP fusion protein attenuates the ER-to-cytosol transport of CTA1. Derlin-1 interacts with CTB and the ER chaperone PDI as assessed by coimmunoprecipitation experiments. An in vitro membrane-binding assay showed that CTB stimulated the unfolded CTA1 chain to bind to the ER membrane. Moreover, intoxication of intact cells with CTB stabilized the degradation of a Derlin-1-dependent substrate, suggesting that CT uses the Derlin-1 pathway. These findings indicate that Derlin-1 facilitates the retro-translocation of CT. CTB may play a role in this process by targeting the holotoxin to Derlin-1, enabling the Derlin-1-bound PDI to unfold the A1 subunit and prepare it for transport."xsd:string |
| http://purl.uniprot.org/citations/18094046 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e07-08-0755"xsd:string |
| http://purl.uniprot.org/citations/18094046 | http://purl.uniprot.org/core/author | "Lencer W.I."xsd:string |
| http://purl.uniprot.org/citations/18094046 | http://purl.uniprot.org/core/author | "Tsai B."xsd:string |
| http://purl.uniprot.org/citations/18094046 | http://purl.uniprot.org/core/author | "Forster M.L."xsd:string |
| http://purl.uniprot.org/citations/18094046 | http://purl.uniprot.org/core/author | "Bernardi K.M."xsd:string |
| http://purl.uniprot.org/citations/18094046 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18094046 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/18094046 | http://purl.uniprot.org/core/pages | "877-884"xsd:string |
| http://purl.uniprot.org/citations/18094046 | http://purl.uniprot.org/core/title | "Derlin-1 facilitates the retro-translocation of cholera toxin."xsd:string |
| http://purl.uniprot.org/citations/18094046 | http://purl.uniprot.org/core/volume | "19"xsd:string |
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