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| Subject | Predicate | Object |
|---|---|---|
| http://purl.uniprot.org/citations/1814007 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1814007 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1814007 | http://www.w3.org/2000/01/rdf-schema#comment | "Venoms of the water cobras, Boulengerina, were assayed for lethality, proteolytic activity and protein content. Boulengerina annulata annulata and B. christyi venoms averaged 89% protein and lacked proteolytic activity. The murine i.p. LD50 of B. a. annulata and B. christyi venoms were 0.143 and 0.120 mg/kg, respectively. Polyvalent antivenom produced by the South African Institute of Medical Research neutralized 575 and 200 LD50 of B. a. annulata and B. christyi venoms/ml antivenom, respectively. Cation exchange chromatography resolved four lethal peaks from B. a. annulata venom and six lethal peaks from B. christyi venom. The major lethal peaks (about 12% of total venom protein) were purified further with molecular sieve chromatography and were characterized as 61 (B. a. annulata toxin) and 62 (B. christyi toxin) residue polypeptides with four half-cystines. Elucidation of the complete amino acid sequences indicated that these toxins belonged to the short-chain class of postsynaptic neurotoxins. Short-chain neurotoxins 1 from B. a. annulata and B. christyi had murine i.p. LD50 of 0.052 and 0.083 mg/kg, respectively, and showed over 80% homology with N. nigricollis alpha toxin. Reverse-phase analysis of another peak present in both venoms resolved a toxin that had an N-terminus identical to B. christyi short-chain neurotoxin 1. These fractions also contained toxins readily separable from the short-chain isotoxin by preparative reverse-phase chromatography. Amino acid sequencing of the first 28 residues indicated that both toxins were long-chain neurotoxins with identical N-termini. The LD50 of long-chain neurotoxins 2 from B. a. annulata and B. christyi venoms were 0.086 and 0.090 mg/kg, respectively. The venoms of these little-known elapids have the lowest LD50 of any African proteroglyph studied thus far and have high concentrations of potent postsynaptic neurotoxins."xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.org/dc/terms/identifier | "doi:10.1016/0041-0101(91)90118-b"xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.org/dc/terms/identifier | "doi:10.1016/0041-0101(91)90118-b"xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/author | "Smith L.A."xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/author | "Smith L.A."xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/author | "Schmidt J.J."xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/author | "Schmidt J.J."xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/author | "Weinstein S.A."xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/author | "Weinstein S.A."xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/name | "Toxicon"xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/name | "Toxicon"xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/pages | "1315-1327"xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/pages | "1315-1327"xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/title | "Lethal toxins and cross-neutralization of venoms from the African water cobras, Boulengerina annulata annulata and Boulengerina christyi."xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/title | "Lethal toxins and cross-neutralization of venoms from the African water cobras, Boulengerina annulata annulata and Boulengerina christyi."xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/volume | "29"xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://purl.uniprot.org/core/volume | "29"xsd:string |
| http://purl.uniprot.org/citations/1814007 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1814007 |
| http://purl.uniprot.org/citations/1814007 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1814007 |
| http://purl.uniprot.org/citations/1814007 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1814007 |
| http://purl.uniprot.org/citations/1814007 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1814007 |