| http://purl.uniprot.org/citations/18161856 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18161856 | http://www.w3.org/2000/01/rdf-schema#comment | "Insulin-like growth factor (IGF-I) has been implicated as a thermoprotective molecule for the preimplantation bovine embryo. Here, it was shown that effects of heat shock (41 degrees C for 15 hr) on induction of apoptosis and reduction in cell number in bovine embryos collected at Day 5 after fertilization were blocked by addition of 100 ng/ml IGF-I at the initiation of heat shock. This action of IGF-I to block heat shock-induced apoptosis was eliminated if embryos were cultured with either a phosphatidylinositol 3-kinase (PI3K) inhibitor (wortmannin) or an Akt inhibitor (1L-6-hydroxymethyl-chiro-inositol 2-(R)-2-o-methyl-3-o-octadecylcarbonate). Immunofluorescence microscopy confirmed the expression of phosphorylated Akt for IGF-I and control embryos. Immunoblotting using an antibody to Akt (phospho S473) indicated increased phosphorylation of Akt in IGF-I-treated embryos. In conclusion, short-term treatment of embryos with IGF-I can block induction of apoptosis caused by heat shock through signaling events requiring PI3K and Akt."xsd:string |
| http://purl.uniprot.org/citations/18161856 | http://purl.org/dc/terms/identifier | "doi:10.1002/mrd.20830"xsd:string |
| http://purl.uniprot.org/citations/18161856 | http://purl.uniprot.org/core/author | "Hansen P.J."xsd:string |
| http://purl.uniprot.org/citations/18161856 | http://purl.uniprot.org/core/author | "Jousan F.D."xsd:string |
| http://purl.uniprot.org/citations/18161856 | http://purl.uniprot.org/core/author | "Oliveira L.J."xsd:string |
| http://purl.uniprot.org/citations/18161856 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18161856 | http://purl.uniprot.org/core/name | "Mol Reprod Dev"xsd:string |
| http://purl.uniprot.org/citations/18161856 | http://purl.uniprot.org/core/pages | "681-688"xsd:string |
| http://purl.uniprot.org/citations/18161856 | http://purl.uniprot.org/core/title | "Short-Term culture of in vitro produced bovine preimplantation embryos with insulin-like growth factor-i prevents heat shock-induced apoptosis through activation of the Phosphatidylinositol 3-Kinase/Akt pathway."xsd:string |
| http://purl.uniprot.org/citations/18161856 | http://purl.uniprot.org/core/volume | "75"xsd:string |
| http://purl.uniprot.org/citations/18161856 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18161856 |
| http://purl.uniprot.org/citations/18161856 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18161856 |
| http://purl.uniprot.org/uniprot/#_Q01314-mappedCitation-18161856 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18161856 |
| http://purl.uniprot.org/uniprot/Q01314 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18161856 |