| http://purl.uniprot.org/citations/18164290 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18164290 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18164290 | http://www.w3.org/2000/01/rdf-schema#comment | "A single cell of wild-type Dictyostelium discoideum forms a visible colony on a plastic dish in several days, but due to enhanced cell migration, amiB-null mutant cells scatter over a large area and do not form noticeable colonies. Here, with an aim to identify genes involved in cell migration, we isolated suppresser mutants of amiB-null mutants that restore the ability to form colonies. From REMI (restriction enzyme-mediated integration)-mutagenized pool of double-mutants, we identified 18 responsible genes from them. These genes can be categorized into several biological processes. One cell line, Sab16 (Suppressor of amiB) was chosen for further analysis, which had a disrupted phospholipase D pldB gene. To confirm the role of pldB gene in cell migration, we knocked out the pldB gene and over-expressed gfp-pldB in wild-type cells. GFP-PLDB localized to plasma membrane and on vesicles, and in migrating cells, at the protruding regions of pseudopodia. Migration speed of vegetative pldB-null cells was reduced to 73% of that of the wild-type. These results suggest that PLDB plays an important role in migration in Dictyostelium cells, and that our screening system is useful for the identification of genes involved in cell migration."xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yexcr.2007.12.002"xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yexcr.2007.12.002"xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/author | "Nagasaki A."xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/author | "Nagasaki A."xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/author | "Uyeda T.Q.P."xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/author | "Uyeda T.Q.P."xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/name | "Exp. Cell Res."xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/name | "Exp. Cell Res."xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/pages | "1136-1146"xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/pages | "1136-1146"xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/title | "Screening of genes involved in cell migration in Dictyostelium."xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/title | "Screening of genes involved in cell migration in Dictyostelium."xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/volume | "314"xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://purl.uniprot.org/core/volume | "314"xsd:string |
| http://purl.uniprot.org/citations/18164290 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18164290 |
| http://purl.uniprot.org/citations/18164290 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18164290 |
| http://purl.uniprot.org/citations/18164290 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18164290 |
| http://purl.uniprot.org/citations/18164290 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18164290 |
| http://purl.uniprot.org/uniprot/Q55DA0 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/18164290 |
| http://purl.uniprot.org/uniprot/Q54WR4 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/18164290 |