| http://purl.uniprot.org/citations/18172012 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18172012 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18172012 | http://www.w3.org/2000/01/rdf-schema#comment | "Histone methylation is crucial for transcriptional regulation and chromatin remodeling. It has been suggested that the SET domain containing protein RE-IIBP (interleukin-5 [IL-5] response element II binding protein) may perform a function in the carcinogenesis of certain tumor types, including myeloma. However, the pathogenic role of RE-IIBP in these diseases remains to be clearly elucidated. In this study, we have conducted an investigation into the relationship between the histone-methylating activity of RE-IIBP and transcriptional regulation. Here, we report that RE-IIBP is up-regulated in the blood cells of leukemia patients, and we characterized the histone H3 lysine 27 (H3-K27) methyltransferase activity of RE-IIBP. Point mutant analysis revealed that SET domain cysteine 483 and arginine 477 are critical residues for the histone methyltransferase (HMTase) activity of RE-IIBP. RE-IIBP also represses basal transcription via histone deacetylase (HDAC) recruitment, which may be mediated by H3-K27 methylation. In the chromatin immunoprecipitation assays, we showed that RE-IIBP overexpression induces histone H3-K27 methylation, HDAC recruitment, and histone H3 hypoacetylation on the IL-5 promoter and represses expression. Conversely, short hairpin RNA-mediated knockdown of RE-IIBP reduces histone H3-K27 methylation and HDAC occupancy around the IL-5 promoter. These data illustrate the important regulatory role of RE-IIBP in transcriptional regulation, thereby pointing to the important role of HMTase activity in carcinogenesis."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.02130-07"xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.02130-07"xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Kim S.M."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Kim S.M."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Kim J.Y."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Kim J.Y."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Kee H.J."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Kee H.J."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Seo S.B."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Seo S.B."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Baek H.J."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Baek H.J."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Kook H."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Kook H."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Eom G.H."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Eom G.H."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Choe N.W."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Choe N.W."xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Kook H.'"xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/author | "Kook H.'"xsd:string |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18172012 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |