| http://purl.uniprot.org/citations/18174166 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18174166 | http://www.w3.org/2000/01/rdf-schema#comment | "Vitronectin and plasminogen activator inhibitor-1 (PAI-1) are important physiological binding partners that work in concert to regulate cellular adhesion, migration, and fibrinolysis. The high affinity binding site for PAI-1 is located within the N-terminal somatomedin B domain of vitronectin; however, several studies have suggested a second PAI-1-binding site within vitronectin. To investigate this secondary site, a vitronectin mutant lacking the somatomedin B domain (rDeltasBVN) was engineered. The short deletion had no effect on heparin-binding, integrin-binding, or cellular adhesion. Binding to the urokinase receptor was completely abolished while PAI-1 binding was still observed, albeit with a lower affinity. Analytical ultracentrifugation on the PAI-1-vitronectin complex demonstrated that increasing NaCl concentration favors 1:1 versus 2:1 PAI-1-vitronectin complexes and hampers formation of higher order complexes, pointing to the contribution of charge-charge interactions for PAI-1 binding to the second site. Furthermore, fluorescence resonance energy transfer between differentially labeled PAI-1 molecules confirmed that two independent molecules of PAI-1 are capable of binding to vitronectin. These results support a model for the assembly of higher order PAI-1-vitronectin complexes via two distinct binding sites in both proteins."xsd:string |
| http://purl.uniprot.org/citations/18174166 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m708017200"xsd:string |
| http://purl.uniprot.org/citations/18174166 | http://purl.uniprot.org/core/author | "Peterson C.B."xsd:string |
| http://purl.uniprot.org/citations/18174166 | http://purl.uniprot.org/core/author | "Minor K.H."xsd:string |
| http://purl.uniprot.org/citations/18174166 | http://purl.uniprot.org/core/author | "Blouse G.E."xsd:string |
| http://purl.uniprot.org/citations/18174166 | http://purl.uniprot.org/core/author | "Schar C.R."xsd:string |
| http://purl.uniprot.org/citations/18174166 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18174166 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/18174166 | http://purl.uniprot.org/core/pages | "10297-10309"xsd:string |
| http://purl.uniprot.org/citations/18174166 | http://purl.uniprot.org/core/title | "A deletion mutant of vitronectin lacking the somatomedin B domain exhibits residual plasminogen activator inhibitor-1-binding activity."xsd:string |
| http://purl.uniprot.org/citations/18174166 | http://purl.uniprot.org/core/volume | "283"xsd:string |
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