| http://purl.uniprot.org/citations/18174280 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18174280 | http://www.w3.org/2000/01/rdf-schema#comment | "The present study was conducted to elucidate the possible molecular mechanisms involved in the antispermatogenic activity of l-CDB-4022, an indenopyridine. In this study 45-d-old male Sprague-Dawley rats were treated with a single oral dose of l-CDB-4022 (2.5 mg/kg) or vehicle, and blood and testes were collected at various time points. The rate of body weight gain was not affected, but a significant loss of testes weight was induced by l-CDB-4022. Serum hormones were assayed using specific RIAs or ELISAs, and testicular protein and RNA were analyzed by Western blotting and RT-PCR, respectively. There was a significant decrease in inhibin B and concomitant increase in FSH in serum from l-CDB-4022-treated rats, but serum levels of activin A, testosterone, and LH were unchanged. Western analysis of testicular lysates from l-CDB-4022-treated rats exhibited phosphorylation of ERK1/2 at 4 h and later time points. Loss of nectin/afadin complex occurred at 48 h, but there was an increase in levels of integrin-beta1, N-cadherin, alpha-catenin, and beta-catenin protein at 24 h and later time points. Increase in expression of Fas ligand and Fas receptor was detected 8 and 24 h after l-CDB-4022 treatment. The ratio of the membrane to soluble form of stem cell factor mRNA was decreased. Immunohistochemical analysis of testicular sections indicated a dramatic disruption of the Sertoli cell microtubule network in l-CDB-4022-treated rats. Collectively, these results suggest that l-CDB-4022 activates the MAPK pathway, reduces expression of prosurvival factors such as the membrane form of stem cell factor, alters expression of Sertoli-germ cell adherens junction proteins, disrupts Sertoli cell microtubule structure, and induces the proapoptotic factor, Fas, culminating in germ cell loss from the seminiferous epithelium."xsd:string |
| http://purl.uniprot.org/citations/18174280 | http://purl.org/dc/terms/identifier | "doi:10.1210/en.2007-1332"xsd:string |
| http://purl.uniprot.org/citations/18174280 | http://purl.uniprot.org/core/author | "Koduri S."xsd:string |
| http://purl.uniprot.org/citations/18174280 | http://purl.uniprot.org/core/author | "Attardi B.J."xsd:string |
| http://purl.uniprot.org/citations/18174280 | http://purl.uniprot.org/core/author | "Hild S.A."xsd:string |
| http://purl.uniprot.org/citations/18174280 | http://purl.uniprot.org/core/author | "Pessaint L."xsd:string |
| http://purl.uniprot.org/citations/18174280 | http://purl.uniprot.org/core/author | "Reel J.R."xsd:string |
| http://purl.uniprot.org/citations/18174280 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18174280 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
| http://purl.uniprot.org/citations/18174280 | http://purl.uniprot.org/core/pages | "1850-1860"xsd:string |
| http://purl.uniprot.org/citations/18174280 | http://purl.uniprot.org/core/title | "Mechanism of action of l-CDB-4022, a potential nonhormonal male contraceptive, in the seminiferous epithelium of the rat testis."xsd:string |
| http://purl.uniprot.org/citations/18174280 | http://purl.uniprot.org/core/volume | "149"xsd:string |
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