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http://purl.uniprot.org/citations/18197420http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18197420http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18197420http://www.w3.org/2000/01/rdf-schema#comment"A gelsolin-related protein was isolated from seminal vesicles of the annelid Lumbricus terrestris. Compared with the isoforms of the gelsolin-related protein previously found in the muscle of the annelid body wall, the isolated protein was assigned to the first isoform (EWAM-P1) because of its electrophoretic mobility, chromatographic elution behaviour, immunological cross-reactivity and identical nucleotide sequence of segments obtained by reverse transcription/polymerase chain reaction. Immunofluorescence studies with smear preparations of developing male germ cells revealed characteristic changes of the local distribution of actin and EWAM-P1 during spermatogenesis. These changes were correlated with the developmental transport processes and structural alterations. F-actin, as revealed by rhodamine-phalloidin staining, formed a toroid-shaped structure in cytoplasmic bridges connecting the germ cells to a central cytophore during the developmental stages. An actin antibody reacting with both G- and F-actin demonstrated that actin was concentrated at the proximal and distal parts of the spermatocytes. EWAM-P1 was also localized in these regions, with intense staining in the distal part of spermatocytes and young spermatids in which the Golgi complex and proacrosome resided. The anti-actin antibody further stained the periphery of the nucleus. This staining gradually reduced during sperm maturation and covered about half of the length of the nucleus in elongated spermatids. Co-localization of EWAM with actin implied a functional significance of this gelsolin-related protein for the rearrangement of the actin cytoskeleton during earthworm spermiogenesis."xsd:string
http://purl.uniprot.org/citations/18197420http://purl.org/dc/terms/identifier"doi:10.1007/s00441-007-0561-9"xsd:string
http://purl.uniprot.org/citations/18197420http://purl.org/dc/terms/identifier"doi:10.1007/s00441-007-0561-9"xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/author"Kruger E."xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/author"Kruger E."xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/author"Hinssen H."xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/author"Hinssen H."xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/author"D'Haese J."xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/author"D'Haese J."xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/name"Cell Tissue Res."xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/name"Cell Tissue Res."xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/pages"141-150"xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/pages"141-150"xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/title"Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris."xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/title"Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris."xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/volume"332"xsd:string
http://purl.uniprot.org/citations/18197420http://purl.uniprot.org/core/volume"332"xsd:string
http://purl.uniprot.org/citations/18197420http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18197420
http://purl.uniprot.org/citations/18197420http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18197420
http://purl.uniprot.org/citations/18197420http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/18197420
http://purl.uniprot.org/citations/18197420http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/18197420