| http://purl.uniprot.org/citations/18208356 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18208356 | http://www.w3.org/2000/01/rdf-schema#comment | "Smurf1, a member of HECT-type E3 ubiquitin ligases, regulates cell polarity and protrusive activity by inducing ubiquitination and subsequent proteasomal degradation of the small GTPase RhoA. We report here that hPEM-2, a guanine nucleotide exchange factor for the small GTPase Cdc42, is a novel target of Smurf1. Pulse-chase labeling and a ubiquitination experiment using MG132, a proteasomal inhibitor, indicate that Smurf1 induces proteasomal degradation of hPEM-2 in cells. GST pull-down assays with heterologously expressed firefly luciferase-fusion proteins that include partial sequences of hPEM-2 reveal that part of the PH domain (residues 318-343) of hPEM-2 is sufficient for binding to Smurf1. In contrast, the hPEM-2 binding domain in Smurf1 was mapped to the C2 domain. Although it has been reported that the binding activities of some C2 domains to target proteins are regulated by Ca2+, Smurf1 interacts with hPEM-2 in a Ca2+-independent manner. Our discovery that hPEM-2 is, in addition to RhoA, a target protein of Smurf1 suggests that Smurf1 plays a crucial role in the spatiotemporal regulation of Rho GTPase family members."xsd:string |
| http://purl.uniprot.org/citations/18208356 | http://purl.org/dc/terms/identifier | "doi:10.1515/bc.2008.036"xsd:string |
| http://purl.uniprot.org/citations/18208356 | http://purl.uniprot.org/core/author | "Nagase T."xsd:string |
| http://purl.uniprot.org/citations/18208356 | http://purl.uniprot.org/core/author | "Ohara O."xsd:string |
| http://purl.uniprot.org/citations/18208356 | http://purl.uniprot.org/core/author | "Yamaguchi K."xsd:string |
| http://purl.uniprot.org/citations/18208356 | http://purl.uniprot.org/core/author | "Ando A."xsd:string |
| http://purl.uniprot.org/citations/18208356 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18208356 | http://purl.uniprot.org/core/name | "Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/18208356 | http://purl.uniprot.org/core/pages | "405-413"xsd:string |
| http://purl.uniprot.org/citations/18208356 | http://purl.uniprot.org/core/title | "Smurf1 directly targets hPEM-2, a GEF for Cdc42, via a novel combination of protein interaction modules in the ubiquitin-proteasome pathway."xsd:string |
| http://purl.uniprot.org/citations/18208356 | http://purl.uniprot.org/core/volume | "389"xsd:string |
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