| http://purl.uniprot.org/citations/18223688 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18223688 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18223688 | http://www.w3.org/2000/01/rdf-schema#comment | "To identify novel methylated gene promoters, we compared differential RNA expression profiles of colorectal cancer (CRC) cell lines with or without treatment of 5-aza-2'-deoxycytidine (5-aza-dC). Out of 1776 genes that were initially 'absent (that is, silenced)' by gene expression array analysis, we selected 163 genes that were increased after 5-aza-dC treatment in at least two of three CRC cell lines. The microarray results were confirmed by Reverse Transcription-PCR, and CpG island of the gene promoters were amplified and sequenced for examination of cancer-specific methylation. Among the genes identified, the deafness, autosomal dominant 5 gene, DFNA5, promoter was found to be methylated in primary tumor tissues with high frequency (65%, 65/100). Quantitative methylation-specific PCR of DFNA5 clearly discriminated primary CRC tissues from normal colon tissues (3%, 3/100). The mRNA expression of DFNA5 in four of five colon cancer tissues was significantly downregulated as compared to normal tissues. Moreover, forced expression of full-length DFNA5 in CRC cell lines markedly decreased the cell growth and colony-forming ability whereas knockdown of DFNA5 increased cell growth in culture. Our data implicate DFNA5 as a novel tumor suppressor gene in CRC and a valuable molecular marker for human cancer."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.onc.1211021"xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.onc.1211021"xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Mori M."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Mori M."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Wu G."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Wu G."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Yamashita K."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Yamashita K."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Chang X."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Chang X."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Kim M.S."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Kim M.S."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Sidransky D."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Sidransky D."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Baek J.H."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Baek J.H."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Nagpal J.K."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Nagpal J.K."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Ratovitski E.A."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Ratovitski E.A."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Trink B."xsd:string |
| http://purl.uniprot.org/citations/18223688 | http://purl.uniprot.org/core/author | "Trink B."xsd:string |