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http://purl.uniprot.org/citations/18234856http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18234856http://www.w3.org/2000/01/rdf-schema#comment"We describe a method for rapid identification of protein kinase substrates. Cdk1 was engineered to accept an ATP analog that allows it to uniquely label its substrates with a bio-orthogonal phosphate analog tag. A highly specific, covalent capture-and-release methodology was developed for rapid purification of tagged peptides derived from labeled substrate proteins. Application of this approach to the discovery of Cdk1-cyclin B substrates yielded identification of >70 substrates and phosphorylation sites. Many of these sites are known to be phosphorylated in vivo, but most of the proteins have not been characterized as Cdk1-cyclin B substrates. This approach has the potential to expand our understanding of kinase-substrate connections in signaling networks."xsd:string
http://purl.uniprot.org/citations/18234856http://purl.org/dc/terms/identifier"doi:10.1073/pnas.0708966105"xsd:string
http://purl.uniprot.org/citations/18234856http://purl.uniprot.org/core/author"Shokat K.M."xsd:string
http://purl.uniprot.org/citations/18234856http://purl.uniprot.org/core/author"Morgan D.O."xsd:string
http://purl.uniprot.org/citations/18234856http://purl.uniprot.org/core/author"Blethrow J.D."xsd:string
http://purl.uniprot.org/citations/18234856http://purl.uniprot.org/core/author"Glavy J.S."xsd:string
http://purl.uniprot.org/citations/18234856http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18234856http://purl.uniprot.org/core/name"Proc Natl Acad Sci U S A"xsd:string
http://purl.uniprot.org/citations/18234856http://purl.uniprot.org/core/pages"1442-1447"xsd:string
http://purl.uniprot.org/citations/18234856http://purl.uniprot.org/core/title"Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates."xsd:string
http://purl.uniprot.org/citations/18234856http://purl.uniprot.org/core/volume"105"xsd:string
http://purl.uniprot.org/citations/18234856http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18234856
http://purl.uniprot.org/citations/18234856http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/18234856
http://purl.uniprot.org/uniprot/#_P0C1Z6-mappedCitation-18234856http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18234856
http://purl.uniprot.org/uniprot/#_P10412-mappedCitation-18234856http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18234856
http://purl.uniprot.org/uniprot/#_Q14157-mappedCitation-18234856http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18234856
http://purl.uniprot.org/uniprot/#_Q14202-mappedCitation-18234856http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18234856
http://purl.uniprot.org/uniprot/#_Q14244-mappedCitation-18234856http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18234856
http://purl.uniprot.org/uniprot/#_Q14247-mappedCitation-18234856http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18234856
http://purl.uniprot.org/uniprot/#_A6NKT7-mappedCitation-18234856http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18234856
http://purl.uniprot.org/uniprot/#_O14715-mappedCitation-18234856http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18234856
http://purl.uniprot.org/uniprot/#_O14773-mappedCitation-18234856http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18234856
http://purl.uniprot.org/uniprot/#_P49915-mappedCitation-18234856http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18234856
http://purl.uniprot.org/uniprot/#_Q14978-mappedCitation-18234856http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18234856