| http://purl.uniprot.org/citations/18237557 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18237557 | http://www.w3.org/2000/01/rdf-schema#comment | "Bone marrow-derived mast cells (BMMCs) contain chondroitin sulfate (CS)-E comprised of GlcA-GalNAc(4SO4) units and GlcA-GalNAc(4,6-SO4) units. GalNAc 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST) transfers sulfate to position 6 of GalNAc(4SO4) residues of CS. On the basis of the specificity of GalNAc4S-6ST, it is thought that CS-E is synthesized in BMMC through the sequential sulfation by chondroitin 4-sulfotransferase (C4ST)-1 and GalNAc4S-6ST. In this paper, we investigated whether GalNAc4S-6ST and C4ST-1 are actually expressed in BMMCs in which CS-E is actively synthesized. As the bone marrow cells differentiate to BMMCs, level of C4ST-1 and GalNAc4S-6ST messages increased, whereas chondroitin 6-sulfotransferase (C6ST)-1 message decreased. In the extract of BMMCs, activity of GalNAc4S-6ST and C4ST but not C6ST were detected. The recombinant mouse GalNAc4S-6ST transferred sulfate to both nonreducing terminal and internal GalNAc(4SO4) residues; the activity toward nonreducing terminal GalNAc(4SO4) was increased with increasing pH. When CS-E synthesized by BMMCs was metabolically labeled with 35SO4 in the presence of bafilomycin A, chloroquine or NH4Cl, the proportion of the nonreducing terminal GalNAc(4,6-SO4) was increased compared with the control, suggesting that GalNAc4S-6ST in BMMC may elaborate CS-E in the intracellular compartment with relatively low pH where sulfation of the internal GalNAc(4SO4) by GalNAc4S-6ST preferentially occurs."xsd:string |
| http://purl.uniprot.org/citations/18237557 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbagen.2008.01.004"xsd:string |
| http://purl.uniprot.org/citations/18237557 | http://purl.uniprot.org/core/author | "Kondo S."xsd:string |
| http://purl.uniprot.org/citations/18237557 | http://purl.uniprot.org/core/author | "Matsumura K."xsd:string |
| http://purl.uniprot.org/citations/18237557 | http://purl.uniprot.org/core/author | "Morisaki T."xsd:string |
| http://purl.uniprot.org/citations/18237557 | http://purl.uniprot.org/core/author | "Kimata K."xsd:string |
| http://purl.uniprot.org/citations/18237557 | http://purl.uniprot.org/core/author | "Habuchi O."xsd:string |
| http://purl.uniprot.org/citations/18237557 | http://purl.uniprot.org/core/author | "Ohtake S."xsd:string |
| http://purl.uniprot.org/citations/18237557 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18237557 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/18237557 | http://purl.uniprot.org/core/pages | "687-695"xsd:string |
| http://purl.uniprot.org/citations/18237557 | http://purl.uniprot.org/core/title | "Expression of sulfotransferases involved in the biosynthesis of chondroitin sulfate E in the bone marrow derived mast cells."xsd:string |
| http://purl.uniprot.org/citations/18237557 | http://purl.uniprot.org/core/volume | "1780"xsd:string |
| http://purl.uniprot.org/citations/18237557 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18237557 |
| http://purl.uniprot.org/citations/18237557 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18237557 |
| http://purl.uniprot.org/uniprot/#_Q91XQ5-mappedCitation-18237557 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18237557 |
| http://purl.uniprot.org/uniprot/Q91XQ5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18237557 |