| http://purl.uniprot.org/citations/18241125 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18241125 | http://www.w3.org/2000/01/rdf-schema#comment | "Trichostatin A (TSA) is the most potent histone deacetylase (HDAC) inhibitor known. We previously reported that treatment of mouse somatic cell nuclear-transferred (SCNT) oocytes with TSA significantly increased the blastocyst rate, blastocyst cell number, and full-term development. How TSA enhances the epigenetic remodeling ability of somatic nuclei and the expression of development-related genes, however, is not known. In the present study, we compared the expression patterns of nine genes involved in chromatin structure and DNA methylation, and seven development-related genes in blastocysts developed from SCNT oocytes treated with and without TSA, and in blastocysts developed in vivo and in vitro using real-time reverse transcription-polymerase chain reaction. In vivo-recovered blastocysts and blastocysts developed from TSA-treated SCNT oocytes exhibited similar expression patterns for Hdac1, 2, and 3, CBP, PCAF, and Dnmt3b genes compared with in vitro-developed blastocysts and blastocysts developed from SCNT oocytes without TSA treatment. There were significantly lower expression levels of Hdac1 and Hdac2 transcripts in TSA-treated and in vivo-recovered blastocysts than in TSA-untreated and in vitro-developed blastocysts. The finding that TSA treatment of SCNT oocytes significantly upregulated Sox2 and cMyc transcripts in blastocysts indicated that both transcripts are TSA-responsive genes. Thus, TSA treatment of mouse SCNT oocytes decreased the expression of chromatin structure- and DNA methylation-related genes, and increased the expression of Sox2 and cMyc genes in blastocysts. Such modifications might be a reason for the high developmental potential of mouse SCNT oocytes treated with TSA."xsd:string |
| http://purl.uniprot.org/citations/18241125 | http://purl.org/dc/terms/identifier | "doi:10.1089/clo.2007.0066"xsd:string |
| http://purl.uniprot.org/citations/18241125 | http://purl.uniprot.org/core/author | "Kato Y."xsd:string |
| http://purl.uniprot.org/citations/18241125 | http://purl.uniprot.org/core/author | "Li X."xsd:string |
| http://purl.uniprot.org/citations/18241125 | http://purl.uniprot.org/core/author | "Tsunoda Y."xsd:string |
| http://purl.uniprot.org/citations/18241125 | http://purl.uniprot.org/core/author | "Tsuji Y."xsd:string |
| http://purl.uniprot.org/citations/18241125 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18241125 | http://purl.uniprot.org/core/name | "Cloning Stem Cells"xsd:string |
| http://purl.uniprot.org/citations/18241125 | http://purl.uniprot.org/core/pages | "133-142"xsd:string |
| http://purl.uniprot.org/citations/18241125 | http://purl.uniprot.org/core/title | "The effects of trichostatin A on mRNA expression of chromatin structure-, DNA methylation-, and development-related genes in cloned mouse blastocysts."xsd:string |
| http://purl.uniprot.org/citations/18241125 | http://purl.uniprot.org/core/volume | "10"xsd:string |
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