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http://purl.uniprot.org/citations/18248670http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18248670http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18248670http://www.w3.org/2000/01/rdf-schema#comment"

Background

The spindle checkpoint delays the onset of anaphase until all sister chromatids are aligned properly at the metaphase plate. To investigate the role san-1, the MAD3 homologue, has in Caenorhabditis elegans embryos we used RNA interference (RNAi) to identify genes synthetic lethal with the viable san-1(ok1580) deletion mutant.

Results

The san-1(ok1580) animal has low penetrating phenotypes including an increased incidence of males, larvae arrest, slow growth, protruding vulva, and defects in vulva morphogenesis. We found that the viability of san-1(ok1580) embryos is significantly reduced when HCP-1 (CENP-F homologue), MDF-1 (MAD-1 homologue), MDF-2 (MAD-2 homologue) or BUB-3 (predicted BUB-3 homologue) are reduced by RNAi. Interestingly, the viability of san-1(ok1580) embryos is not significantly reduced when the paralog of HCP-1, HCP-2, is reduced. The phenotype of san-1(ok1580);hcp-1(RNAi) embryos includes embryonic and larval lethality, abnormal organ development, and an increase in abnormal chromosome segregation (aberrant mitotic nuclei, anaphase bridging). Several of the san-1(ok1580);hcp-1(RNAi) animals displayed abnormal kinetochore (detected by MPM-2) and microtubule structure. The survival of mdf-2(RNAi);hcp-1(RNAi) embryos but not bub-3(RNAi);hcp-1(RNAi) embryos was also compromised. Finally, we found that san-1(ok1580) and bub-3(RNAi), but not hcp-1(RNAi) embryos, were sensitive to anoxia, suggesting that like SAN-1, BUB-3 has a functional role as a spindle checkpoint protein.

Conclusion

Together, these data suggest that in the C. elegans embryo, HCP-1 interacts with a subset of the spindle checkpoint pathway. Furthermore, the fact that san-1(ok1580);hcp-1(RNAi) animals had a severe viability defect whereas in the san-1(ok1580);hcp-2(RNAi) and san-1(ok1580);hcp-2(ok1757) animals the viability defect was not as severe suggesting that hcp-1 and hcp-2 are not completely redundant."xsd:string
http://purl.uniprot.org/citations/18248670http://purl.org/dc/terms/identifier"doi:10.1186/1747-1028-3-6"xsd:string
http://purl.uniprot.org/citations/18248670http://purl.org/dc/terms/identifier"doi:10.1186/1747-1028-3-6"xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/author"Stewart A.M."xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/author"Stewart A.M."xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/author"Hajeri V.A."xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/author"Hajeri V.A."xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/author"Moore L.L."xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/author"Moore L.L."xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/author"Padilla P.A."xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/author"Padilla P.A."xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/name"Cell Div."xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/name"Cell Div."xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/pages"6"xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/pages"6"xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/title"Genetic analysis of the spindle checkpoint genes san-1, mdf-2, bub-3 and the CENP-F homologues hcp-1 and hcp-2 in Caenorhabditis elegans."xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/title"Genetic analysis of the spindle checkpoint genes san-1, mdf-2, bub-3 and the CENP-F homologues hcp-1 and hcp-2 in Caenorhabditis elegans."xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/volume"3"xsd:string
http://purl.uniprot.org/citations/18248670http://purl.uniprot.org/core/volume"3"xsd:string
http://purl.uniprot.org/citations/18248670http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18248670
http://purl.uniprot.org/citations/18248670http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18248670
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