| http://purl.uniprot.org/citations/18260642 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18260642 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18260642 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/18260642 | http://www.w3.org/2000/01/rdf-schema#comment | "Coenzyme F 420 is a hydride carrier cofactor functioning in methanogenesis. One step in the biosynthesis of coenzyme F 420 involves the coupling of 2-phospho-l-lactate (LP) to 7,8-didemethyl-8-hydroxy-5-deazaflavin, the F 420 chromophore. This condensation requires an initial activation of 2-phospho-l-lactate through a pyrophosphate linkage to GMP. Bioinformatic analysis identified an uncharacterized archaeal protein in the Methanocaldococcus jannaschii genome, MJ0887, which could be involved in this transformation. The predicted MJ0887-derived protein has domain similarity with other known nucleotidyl transferases. The MJ0887 gene was cloned and overexpressed, and the purified protein was found to catalyze the formation of lactyl-2-diphospho-5'-guanosine from LP and GTP. Kinetic constants were determined for the MJ0887-derived protein with both LP and GTP substrates and are as follows: V max = 3 micromol min (-1) mg (-1), GTP K M (app) = 56 microM, and k cat/ K M (app) = 2 x 10 (4) M (-1) s (-1) and LP K M (app) = 36 microM, and k cat/ K M (app) = 4 x 10 (4) M (-1) s (-1). The MJ0887 gene product has been designated CofC to indicate its involvement in the third step of coenzyme F 420 biosynthesis."xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi702475t"xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi702475t"xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi702475t"xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/author | "White R.H."xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/author | "White R.H."xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/author | "Xu H."xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/author | "Xu H."xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/author | "Grochowski L.L."xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/author | "Grochowski L.L."xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/pages | "3033-3037"xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/pages | "3033-3037"xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/title | "Identification and characterization of the 2-phospho-L-lactate guanylyltransferase involved in coenzyme F420 biosynthesis."xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/title | "Identification and characterization of the 2-phospho-L-lactate guanylyltransferase involved in coenzyme F420 biosynthesis."xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/volume | "47"xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://purl.uniprot.org/core/volume | "47"xsd:string |
| http://purl.uniprot.org/citations/18260642 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18260642 |
| http://purl.uniprot.org/citations/18260642 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18260642 |