| http://purl.uniprot.org/citations/18273061 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18273061 | http://www.w3.org/2000/01/rdf-schema#comment | "The c-Cbl tyrosine kinase binding domain (Cbl-TKB), essentially an 'embedded' SH2 domain, has a critical role in targeting proteins for ubiquitination. To address how this domain can bind to disparate recognition mofits and to determine whether this results in variations in substrate-binding affinity, we compared crystal structures of the Cbl-TKB domain complexed with phosphorylated peptides of Sprouty2, Sprouty4, epidermal growth factor receptor, Syk, and c-Met receptors and validated the binding with point-mutational analyses using full-length proteins. An obligatory, intrapeptidyl H-bond between the phosphotyrosine and the conserved asparagine or adjacent arginine is essential for binding and orients the peptide into a positively charged pocket on c-Cbl. Surprisingly, c-Met bound to Cbl in the reverse direction, which is unprecedented for SH2 domain binding. The necessity of this intrapeptidyl H-bond was confirmed with isothermal titration calorimetry experiments that also showed Sprouty2 to have the highest binding affinity to c-Cbl; this may enable the selective sequestration of c-Cbl from other target proteins."xsd:string |
| http://purl.uniprot.org/citations/18273061 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2008.18"xsd:string |
| http://purl.uniprot.org/citations/18273061 | http://purl.uniprot.org/core/author | "Guy G.R."xsd:string |
| http://purl.uniprot.org/citations/18273061 | http://purl.uniprot.org/core/author | "Sun Q."xsd:string |
| http://purl.uniprot.org/citations/18273061 | http://purl.uniprot.org/core/author | "Sivaraman J."xsd:string |
| http://purl.uniprot.org/citations/18273061 | http://purl.uniprot.org/core/author | "Ng C."xsd:string |
| http://purl.uniprot.org/citations/18273061 | http://purl.uniprot.org/core/author | "Buschdorf J.P."xsd:string |
| http://purl.uniprot.org/citations/18273061 | http://purl.uniprot.org/core/author | "Jackson R.A."xsd:string |
| http://purl.uniprot.org/citations/18273061 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18273061 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/18273061 | http://purl.uniprot.org/core/pages | "804-816"xsd:string |
| http://purl.uniprot.org/citations/18273061 | http://purl.uniprot.org/core/title | "Structural basis for a novel intrapeptidyl H-bond and reverse binding of c-Cbl-TKB domain substrates."xsd:string |
| http://purl.uniprot.org/citations/18273061 | http://purl.uniprot.org/core/volume | "27"xsd:string |
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