| http://purl.uniprot.org/citations/18276161 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18276161 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18276161 | http://www.w3.org/2000/01/rdf-schema#comment | "Betanodavirus protein alpha induces cell apoptosis or secondary necrosis by a poorly understood process. In the present work, red spotted grouper nervous necrosis virus (RGNNV) RNA 2 was cloned and transfected into tissue culture cells (GF-1) which then underwent apoptosis or post-apoptotic necrosis. In the early apoptotic stage, progressive phosphatidylserine externalization was evident at 24h post-transfection (p.t.) by Annexin V-FLUOS staining. TUNEL assay revealed apoptotic cells at 24-72 h p.t, after which post-apoptotic necrotic cells were identified by acridine orange/ethidium bromide dual dye staining from 48 to 72 h p.t. Protein alpha induced progressive loss of mitochondrial membrane potential (MMP) which was detected in RNA2-transfected GF-1 cells at 24, 48, and 72 h p.t., which correlated with cytochrome c release, especially at 72 h p.t. To assess the effect of zfBcl-xL on cell death, RNA2-transfected cells were co-transfected with zfBcl-x(L). Co-transfection of GF-1 cells prevented loss of MMP at 24 h and 48 h p.t. and blocked initiator caspase-8 and effector caspase-3 activation at 48 h p.t. We conclude that RGNNV protein alpha induces apoptosis followed by secondary necrotic cell death through a mitochondria-mediated death pathway and activation of caspases-8 and -3."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.fsi.2008.01.001"xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.fsi.2008.01.001"xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Wu H.C."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Wu H.C."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Chen M.C."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Chen M.C."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Wu J.L."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Wu J.L."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Gong H.Y."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Gong H.Y."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Chiu C.S."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Chiu C.S."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Lu M.W."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Lu M.W."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Hong J.R."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/author | "Hong J.R."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/name | "Fish Shellfish Immunol."xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/name | "Fish Shellfish Immunol"xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/pages | "436-449"xsd:string |
| http://purl.uniprot.org/citations/18276161 | http://purl.uniprot.org/core/pages | "436-449"xsd:string |