| http://purl.uniprot.org/citations/18283539 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18283539 | http://www.w3.org/2000/01/rdf-schema#comment | "DFF40/CAD endonuclease is primarily responsible for internucleosomal DNA cleavage during the terminal stages of apoptosis. The nuclease specifically introduces DNA double strand breaks into chromatin substrates. Here we performed a detailed study on the specificity of the nuclease using synthetic single-stranded and double-stranded ribo- and deoxyribo-oligonucleotides as substrates. We have found that neither single-stranded DNA, single-stranded RNA, double-stranded RNA nor RNA-DNA heteroduplexes are cleaved by the DFF40/CAD nuclease. Noteworthy, all types of oligonucleotides that are not cleaved by the nuclease inhibit cleavage of double-stranded DNA. We have also observed that in cells undergoing apoptosis in vivo neither the activation of DFF40/CAD nor oligonucleosomal chromatin fragmentation was temporally correlated with either total cellular or nuclear RNA degradation. We conclude that DFF40/CAD is exclusively specific for double-stranded DNA."xsd:string |
| http://purl.uniprot.org/citations/18283539 | http://purl.org/dc/terms/identifier | "doi:10.1007/s10495-008-0183-z"xsd:string |
| http://purl.uniprot.org/citations/18283539 | http://purl.uniprot.org/core/author | "Widlak P."xsd:string |
| http://purl.uniprot.org/citations/18283539 | http://purl.uniprot.org/core/author | "Hanus J."xsd:string |
| http://purl.uniprot.org/citations/18283539 | http://purl.uniprot.org/core/author | "Kalinowska-Herok M."xsd:string |
| http://purl.uniprot.org/citations/18283539 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18283539 | http://purl.uniprot.org/core/name | "Apoptosis"xsd:string |
| http://purl.uniprot.org/citations/18283539 | http://purl.uniprot.org/core/pages | "377-382"xsd:string |
| http://purl.uniprot.org/citations/18283539 | http://purl.uniprot.org/core/title | "The major apoptotic endonuclease DFF40/CAD is a deoxyribose-specific and double-strand-specific enzyme."xsd:string |
| http://purl.uniprot.org/citations/18283539 | http://purl.uniprot.org/core/volume | "13"xsd:string |
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