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| http://purl.uniprot.org/citations/18294864 | http://www.w3.org/2000/01/rdf-schema#comment | "Receptor activation by IL5 and GM-CSF is a sequential process that depends on their interaction with a cytokine-specific subunit alpha and recruitment of a common signaling subunit beta (betac). In order to elucidate the assembly dynamics of these receptor subunits, we performed kinetic interaction analysis of the cytokine-receptor complex formation by a surface plasmon resonance biosensor. Using the extracellular domains of receptor fused with C-terminal V5-tag, we developed an assay method to co-anchor alpha and betac subunits on the biosensor surface. We demonstrated that dissociation of the cytokine-receptor complexes was slower when both subunits were co-anchored on the biosensor surface than when alpha subunit alone was anchored. The slow-dissociation effect of betac had a similar impact on GM-CSF receptor stabilization to that of IL5. The effects were abolished by alanine replacement of either Tyr18 or Tyr344 residue in betac, which together constitute key parts of a cytokine binding epitope. The data argue that betac plays an important role in preventing the ligand-receptor complexes from rapidly dissociating. This slow-dissociation effect of betac explains how, when multiple betac cytokine receptor alpha subunits are present on the same cell surface, selective betac usage can be controlled by sequestration in stabilized cytokine-alpha-betac complexes."xsd:string |
| http://purl.uniprot.org/citations/18294864 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cyto.2007.12.010"xsd:string |
| http://purl.uniprot.org/citations/18294864 | http://purl.uniprot.org/core/author | "Ishino T."xsd:string |
| http://purl.uniprot.org/citations/18294864 | http://purl.uniprot.org/core/author | "Zaks-Zilberman M."xsd:string |
| http://purl.uniprot.org/citations/18294864 | http://purl.uniprot.org/core/author | "Harrington A.E."xsd:string |
| http://purl.uniprot.org/citations/18294864 | http://purl.uniprot.org/core/author | "Chaiken I."xsd:string |
| http://purl.uniprot.org/citations/18294864 | http://purl.uniprot.org/core/author | "Scibek J.J."xsd:string |
| http://purl.uniprot.org/citations/18294864 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18294864 | http://purl.uniprot.org/core/name | "Cytokine"xsd:string |
| http://purl.uniprot.org/citations/18294864 | http://purl.uniprot.org/core/pages | "179-190"xsd:string |
| http://purl.uniprot.org/citations/18294864 | http://purl.uniprot.org/core/title | "Slow-dissociation effect of common signaling subunit beta c on IL5 and GM-CSF receptor assembly."xsd:string |
| http://purl.uniprot.org/citations/18294864 | http://purl.uniprot.org/core/volume | "42"xsd:string |
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