| http://purl.uniprot.org/citations/18331345 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18331345 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18331345 | http://www.w3.org/2000/01/rdf-schema#comment | "Hepatoma-derived growth factor is a nuclear targeted mitogen containing a PWWP domain that mediates binding to DNA. To date, almost nothing is known about the molecular mechanisms of the functions of hepatoma-derived growth factor, its routes of secretion and internalization or post-translational modifications. In the present study, we show for the first time that hepatoma-derived growth factor is modified by the covalent attachment of small ubiquitin-related modifier 1 (SUMO-1), a post-translational modification with regulatory functions for an increasing number of proteins. Using a basal SUMOylation system in Escherichia coli followed by a MALDI-TOF-MS based peptide analysis, we identified the lysine residue SUMOylated located in the N-terminal part of the protein adjacent to the PWWP domain. Surprisingly, this lysine residue is not part of the consensus motif described for SUMOylation. With a series of hepatoma-derived growth factor mutants, we then confirmed that this unusual location is also used in mammalian cells and that SUMOylation of hepatoma-derived growth factor takes place in the nucleus. Finally, we demonstrate that SUMOylated hepatoma-derived growth factor is not binding to chromatin, in contrast to its unSUMOylated form. These observations potentially provide new perspectives for a better understanding of the functions of hepatoma-derived growth factor."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1742-4658.2008.06303.x"xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1742-4658.2008.06303.x"xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Gupta S."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Gupta S."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Kelm S."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Kelm S."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Niedenthal R."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Niedenthal R."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Okaz E."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Okaz E."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Jakobs A."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Jakobs A."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Franken S."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Franken S."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Dietz F."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Dietz F."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Thakar K."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/author | "Thakar K."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/name | "FEBS J."xsd:string |
| http://purl.uniprot.org/citations/18331345 | http://purl.uniprot.org/core/name | "FEBS J."xsd:string |