| http://purl.uniprot.org/citations/18375390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18375390 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18375390 | http://www.w3.org/2000/01/rdf-schema#comment | "The ascidian voltage-sensing phosphatase (Ci-VSP) consists of the voltage sensor domain (VSD) and a cytoplasmic phosphatase region that has significant homology to the phosphatase and tensin homolog deleted on chromosome TEN (PTEN). The phosphatase activity of Ci-VSP is modified by the conformational change of the VSD. In many proteins, two protein modules are bidirectionally coupled, but it is unknown whether the phosphatase domain could affect the movement of the VSD in VSP. We addressed this issue by whole-cell patch recording of gating currents from a teleost VSP (Dr-VSP) cloned from Danio rerio expressed in tsA201 cells. Replacement of a critical cysteine residue, in the phosphatase active center of Dr-VSP, by serine sharpened both ON- and OFF-gating currents. Similar changes were produced by treatment with phosphatase inhibitors, pervanadate and orthovanadate, that constitutively bind to cysteine in the active catalytic center of phosphatases. The distinct kinetics of gating currents dependent on enzyme activity were not because of altered phosphatidylinositol 4,5-bisphosphate levels, because the kinetics of gating current did not change by depletion of phosphatidylinositol 4,5-bisphosphate, as reported by coexpressed KCNQ2/3 channels. These results indicate that the movement of the VSD is influenced by the enzymatic state of the cytoplasmic domain, providing an important clue for understanding mechanisms of coupling between the VSD and its effector."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.M706184200"xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m706184200"xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Iwasaki H."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Iwasaki H."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Okamura Y."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Okamura Y."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Nagayama K."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Nagayama K."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Higashijima S."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Higashijima S."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Hossain M.I."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Hossain M.I."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Okochi Y."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Okochi Y."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Chahine M."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/author | "Chahine M."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/pages | "18248-18259"xsd:string |
| http://purl.uniprot.org/citations/18375390 | http://purl.uniprot.org/core/pages | "18248-18259"xsd:string |