| http://purl.uniprot.org/citations/18390877 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18390877 | http://www.w3.org/2000/01/rdf-schema#comment | "Fibrin deposition was universal in the lungs of SARS patients and fgl2 prothrombinase gene, a novel procoagulant, was demonstrated to express highly in a clinically relevant SARS model. To investigate whether and which structural protein of SARS-CoV induced transcription of hfgl2 prothrombinase gene, three eukaryotic expression plasmids expressing nucleocapsid protein (N), membrane protein (M) and spike protein 2 (S2) of SARS-CoV were co-transfected with hfgl2 promoter luciferase-reporter plasmids and beta-galactosidase plasmid in CHO cells, respectively. M, N and S2 protein of SARS-CoV were detected by western blotting and immunohistochemistry analysis. Further assays demonstrated that expression of hfgl2 gene was related with N protein, but not with M or S2 protein in THP-1 cells and Vero cells. N protein significantly induced functional procoagulant activity in comparison with control group. Luciferase assay showed that N protein of SARS-CoV could activate the transcription of hfgl2 promoter compared with the pcDNA3.1 empty vector. Site-directed mutagenesis and EMSA assay further demonstrated that transcription factor C/EBP alpha band with its cognate cis-element in hfgl2 promoter. The results showed that N protein of SARS-CoV induced hfgl2 gene transcription dependent on the transcription factor C/EBP alpha, which maybe contribute to the development of thrombosis in SARS."xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.org/dc/terms/identifier | "doi:10.1093/jb/mvn042"xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/author | "Huang Y."xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/author | "Han M."xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/author | "Li W."xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/author | "Luo X."xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/author | "Yao H."xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/author | "Wang Z."xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/author | "Yan W."xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/author | "Zhou Y."xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/author | "Hou J."xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/author | "Ning Q."xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/author | "Xi D."xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/name | "J Biochem"xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/pages | "51-62"xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/title | "The nucleocapsid protein of SARS-CoV induces transcription of hfgl2 prothrombinase gene dependent on C/EBP alpha."xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://purl.uniprot.org/core/volume | "144"xsd:string |
| http://purl.uniprot.org/citations/18390877 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18390877 |
| http://purl.uniprot.org/citations/18390877 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18390877 |
| http://purl.uniprot.org/uniprot/#_A4D1B8-mappedCitation-18390877 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18390877 |
| http://purl.uniprot.org/uniprot/#_Q53GD2-mappedCitation-18390877 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18390877 |
| http://purl.uniprot.org/uniprot/#_Q14314-mappedCitation-18390877 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18390877 |
| http://purl.uniprot.org/uniprot/#_Q8WWE4-mappedCitation-18390877 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18390877 |