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http://purl.uniprot.org/citations/18397254http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18397254http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18397254http://www.w3.org/2000/01/rdf-schema#comment"

Aims

To characterize the novel bacteriocin produced by Enterococcus durans.

Methods and results

Enterococcus durans QU 49 was isolated from carrot and expressed bactericidal activity over 20-43 degrees C. Bacteriocins were purified to homogeneity using the three-step purification method, one of which, termed durancin TW-49M, was an enterocin B-homologous peptide with most identical residues occurring in the N-terminus. Durancin TW-49M was more tolerant in acidic than in alkali. DNA sequencing analysis revealed durancin TW-49M was translated as a prepeptide of the double-glycine type. Durancin TW-49M and enterocin B expressed similar antimicrobial spectra, in which no significant variation due to the diversity in their C-termini was observed.

Conclusions

Durancin TW-49M, a novel nonpediocin-like class II bacteriocin, was characterized to the amino acid and genetic levels. The diverse C-terminal parts of durancin TW-49M and enterocin B were hardly to be suggested as the place determining the target cell specificity.

Significance and impact of the study

This is the first and comprehensive study of a novel bacteriocin produced by Ent. durans. The high homology at the N-terminal halves between durancin TW-49M and enterocin B makes them suitable to study the structure-function relationship of bacteriocins and their immunity proteins."xsd:string
http://purl.uniprot.org/citations/18397254http://purl.org/dc/terms/identifier"doi:10.1111/j.1365-2672.2008.03798.x"xsd:string
http://purl.uniprot.org/citations/18397254http://purl.org/dc/terms/identifier"doi:10.1111/j.1365-2672.2008.03798.x"xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/author"Nakayama J."xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/author"Nakayama J."xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/author"Sonomoto K."xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/author"Sonomoto K."xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/author"Zendo T."xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/author"Zendo T."xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/author"Hu C.B."xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/author"Hu C.-B."xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/name"J. Appl. Microbiol."xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/name"J Appl Microbiol"xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/pages"681-690"xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/pages"681-690"xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/title"Description of durancin TW-49M, a novel enterocin B-homologous bacteriocin in carrot-isolated Enterococcus durans QU 49."xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/title"Description of durancin TW-49M, a novel enterocin B-homologous bacteriocin in carrot-isolated Enterococcus durans QU 49."xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/volume"105"xsd:string
http://purl.uniprot.org/citations/18397254http://purl.uniprot.org/core/volume"105"xsd:string
http://purl.uniprot.org/citations/18397254http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18397254
http://purl.uniprot.org/citations/18397254http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18397254
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