| http://purl.uniprot.org/citations/18402598 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18402598 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18402598 | http://www.w3.org/2000/01/rdf-schema#comment | "A combinatorial Fab library was constructed in pComb3H phagemid vectors, using RNA from peripheral blood lymphocytes of a healthy volunteer who had recovered from an influenza A virus infection. The library contained approximately 1.3 x 10(8)E. coli transformants. Bio-panning was carried out against an influenza vaccine containing components of influenza A/New Caledonia/20/99 (H1N1), A/Panama/2007/99 (H3N2), and B/Shandong/7/97 for the enrichment of phages displaying human Fab specific to the viral proteins. E. coli transformed with IF1A11, 1 of 94 randomly selected clones, displayed a human Fab antibody molecule (FabIF1A11) with efficient neutralizing activity against H3N2 influenza A virus strains. The purified FabIF1A11 demonstrated neutralizing activity against A/Okayama/6/01 (H3N2) and A/Kitakyushu/159/93 (H3N2) with 50% plaque reduction neutralization titers of 0.11 microg/ml (2.2 nM) and 1.4 microg/ml (28 nM) respectively. However, FabIF1A11 did not show neutralizing activity against the influenza A virus strain A/USSR/77 (H1N1) or the influenza B virus strain B/Kanagawa/73, even at a concentration of 20 microg/ml (400 nM). The Kd of FabIF1A11 was calculated as 3.6 x 10(-9) M. FabIF1A11 was estimated to recognize a conformational epitope on the hemagglutinin of A/Okayama/6/01 (H3N2). The human monoclonal Fab product FabIF1A11 may have potential as a therapeutic or short-term prophylactic molecule for humans with influenza A H3N2 infection."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1348-0421.2008.00025.x"xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1348-0421.2008.00025.x"xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/author | "Uechi G."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/author | "Uechi G."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/author | "Shibuya T."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/author | "Shibuya T."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/author | "Nishizono A."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/author | "Nishizono A."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/author | "Yamashiro T."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/author | "Yamashiro T."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/author | "Ohuchi M."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/author | "Ohuchi M."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/author | "Masaike Y."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/author | "Masaike Y."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/name | "Microbiol. Immunol."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/name | "Microbiol Immunol"xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/pages | "162-170"xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/pages | "162-170"xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/title | "Identification of a human monoclonal Fab with neutralizing activity against H3N2 influenza A strain from a newly constructed human Fab library."xsd:string |
| http://purl.uniprot.org/citations/18402598 | http://purl.uniprot.org/core/title | "Identification of a human monoclonal Fab with neutralizing activity against H3N2 influenza A strain from a newly constructed human Fab library."xsd:string |