| http://purl.uniprot.org/citations/18418385 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18418385 | http://www.w3.org/2000/01/rdf-schema#comment | "The human Mediator complex is generally required for expression of protein-coding genes. Here, we show that the GCN5L acetyltransferase stably associates with Mediator together with the TRRAP polypeptide. Yet, contrary to expectations, TRRAP/GCN5L does not associate with the transcriptionally active core Mediator but rather with Mediator that contains the cdk8 subcomplex. Consequently, this derivative 'T/G-Mediator' complex does not directly activate transcription in a reconstituted human transcription system. However, within T/G-Mediator, cdk8 phosphorylates serine-10 on histone H3, which in turn stimulates H3K14 acetylation by GCN5L within the complex. Tandem phosphoacetylation of H3 correlates with transcriptional activation, and ChIP assays demonstrate co-occupancy of T/G-Mediator components at several activated genes in vivo. Moreover, cdk8 knockdown causes substantial reduction of global H3 phosphoacetylation, suggesting that T/G-Mediator is a major regulator of this H3 mark. Cooperative H3 modification provides a mechanistic basis for GCN5L association with cdk8-Mediator and also identifies a biochemical means by which cdk8 can indirectly activate gene expression. Indeed our results suggest that T/G-Mediator directs early events-such as modification of chromatin templates-in transcriptional activation."xsd:string |
| http://purl.uniprot.org/citations/18418385 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2008.78"xsd:string |
| http://purl.uniprot.org/citations/18418385 | http://purl.uniprot.org/core/author | "Espinosa J.M."xsd:string |
| http://purl.uniprot.org/citations/18418385 | http://purl.uniprot.org/core/author | "Taatjes D.J."xsd:string |
| http://purl.uniprot.org/citations/18418385 | http://purl.uniprot.org/core/author | "Meyer K.D."xsd:string |
| http://purl.uniprot.org/citations/18418385 | http://purl.uniprot.org/core/author | "Knuesel M.T."xsd:string |
| http://purl.uniprot.org/citations/18418385 | http://purl.uniprot.org/core/author | "Donner A.J."xsd:string |
| http://purl.uniprot.org/citations/18418385 | http://purl.uniprot.org/core/author | "York A.G."xsd:string |
| http://purl.uniprot.org/citations/18418385 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18418385 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/18418385 | http://purl.uniprot.org/core/pages | "1447-1457"xsd:string |
| http://purl.uniprot.org/citations/18418385 | http://purl.uniprot.org/core/title | "Cooperative activity of cdk8 and GCN5L within Mediator directs tandem phosphoacetylation of histone H3."xsd:string |
| http://purl.uniprot.org/citations/18418385 | http://purl.uniprot.org/core/volume | "27"xsd:string |
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