| http://purl.uniprot.org/citations/18420581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18420581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18420581 | http://www.w3.org/2000/01/rdf-schema#comment | "To resolve primary (glycosylation-assisted) from secondary (glycosylation-independent) quality control steps in the biosynthesis of HLA (human leukocyte antigen) class I glycoproteins, the unique N-linked glycosylation site of the HLA-Cw1 heavy chain was deleted by site-directed mutagenesis. The non-glycosylated Cw1S88G mutant was characterized by flow cytometry, pulse-chase, co-immunoprecipitation, and in vitro assembly assays with synthetic peptide ligands upon transfection in 721.221 and 721.220 cells. The former provide a full set of primary as well as secondary chaperoning interactions, whereas the latter are unable to perform secondary quality control (e.g. proper class I assembly with peptide antigens) as a result of a functional defect of the HLA-dedicated chaperone tapasin. In both transfectants, Cw1S88G displayed a loss/weakening in its generic chaperoning interaction with calreticulin and/or ERp57 and became redistributed toward calnexin, known to bind the most unfolded class I conformers. Despite this, and quite unexpectedly, a weak interaction with the HLA-dedicated chaperone TAP was selectively retained in 721.221. In addition, the ordered, stepwise acquisition of thermal stability/peptide binding was disrupted, resulting in a heterogeneous ensemble of Cw1S88G conformers with unorthodox and unprecedented peptide assembly features. Because a lack of glycosylation and a lack of tapasin-assisted peptide loading have distinct, complementary, and additive effects, the former is separable from (and upstream of) the latter, e.g. primary quality control is suggested to supervise a crucial, generic folding step preliminary to the acquisition of peptide receptivity."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m709175200"xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m709175200"xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Colonna M."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Colonna M."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Setini A."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Setini A."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Fruci D."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Fruci D."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Giacomini P."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Giacomini P."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Sibilio L."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Sibilio L."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Lo Monaco E."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Lo Monaco E."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Martayan A."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Martayan A."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Tremante E."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/author | "Tremante E."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/18420581 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |