| http://purl.uniprot.org/citations/18425141 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18425141 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18425141 | http://www.w3.org/2000/01/rdf-schema#comment | "Cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)) is required for translation and is typically synthesized by cysteinyl-tRNA synthetase (CysRS). However, Methanocaldococcus jannaschii synthesizes Cys-tRNA(Cys) by an indirect pathway, whereby O-phosphoseryl-tRNA synthetase (SepRS) acylates tRNA(Cys) with phosphoserine (Sep), and Sep-tRNA-Cys-tRNA synthase (SepCysS) converts the tRNA-bound phosphoserine to cysteine. We show here that M. jannaschii SepRS differs from CysRS by recruiting the m1G37 modification as a determinant for aminoacylation, and in showing limited discrimination against mutations of conserved nucleotides. Kinetic and binding measurements show that both SepRS and SepCysS bind the reaction intermediate Sep-tRNA(Cys) tightly, and these two enzymes form a stable binary complex that promotes conversion of the intermediate to the product and sequesters the intermediate from binding to elongation factor EF-1alpha or infiltrating into the ribosome. These results highlight the importance of the protein binary complex for efficient synthesis of Cys-tRNA(Cys)."xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.org/dc/terms/identifier | "doi:10.1038/nsmb.1423"xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.org/dc/terms/identifier | "doi:10.1038/nsmb.1423"xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/author | "Liu C."xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/author | "Liu C."xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/author | "Slater S."xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/author | "Slater S."xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/author | "Hou Y.-M."xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/author | "Hou Y.-M."xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/author | "Zhang C.-M."xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/author | "Zhang C.-M."xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/name | "Nat. Struct. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/name | "Nat. Struct. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/pages | "507-514"xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/pages | "507-514"xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/title | "Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys)."xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/title | "Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys)."xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/18425141 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18425141 |
| http://purl.uniprot.org/citations/18425141 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18425141 |