| http://purl.uniprot.org/citations/18434434 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18434434 | http://www.w3.org/2000/01/rdf-schema#comment | "Previous work suggested that altered Ca(2+) homeostasis might contribute to dysfunction of nebulin-free muscle, as gene expression analysis revealed that the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA)-inhibitor sarcolipin (SLN) is up-regulated >70-fold in nebulin knockout mice, and here we tested this proposal. We investigated SLN protein expression in nebulin-free and wild-type skeletal muscle, as well as expression of other Ca(2+)-handling proteins. Ca(2+) uptake capacity was determined in isolated sarcoplasmic reticulum vesicles and in intact myofibers by measuring Ca(2+) transients. Muscle contractile performance was determined in skinned muscle activated with exogenous Ca(2+), as well as in electrically stimulated intact muscle. We found profound up-regulation of SLN protein in nebulin-free skeletal muscle, whereas expression of other Ca(2+)-handling proteins was not (calsequestrin and phospholamban) or was minimally (SERCA) affected. Speed of Ca(2+) uptake was >3-fold decreased in sarcoplasmic reticulum vesicles isolated from nebulin-free muscle as well as in nebulin-free intact myofibers. Ca(2+)-activated stress in skinned muscle and stress produced by intact nebulin-free muscle were reduced to a similar extent compared with wild type. Half-relaxation time was significantly longer in nebulin-free compared with wild-type muscle. Thus, the present study demonstrates for the first time that nebulin might also be involved in physiological Ca(2+) handling of the SR-myofibrillar system."xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.org/dc/terms/identifier | "doi:10.1096/fj.07-104372"xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/author | "Fong C."xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/author | "Wuytack F."xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/author | "Periasamy M."xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/author | "Granzier H."xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/author | "Labeit S."xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/author | "Witt C.C."xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/author | "Vangheluwe P."xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/author | "Babu G.J."xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/author | "Ottenheijm C.A."xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/name | "FASEB J"xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/pages | "2912-2919"xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/title | "Sarcoplasmic reticulum calcium uptake and speed of relaxation are depressed in nebulin-free skeletal muscle."xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://purl.uniprot.org/core/volume | "22"xsd:string |
| http://purl.uniprot.org/citations/18434434 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18434434 |
| http://purl.uniprot.org/citations/18434434 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18434434 |
| http://purl.uniprot.org/uniprot/#_A2AQA9-mappedCitation-18434434 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18434434 |
| http://purl.uniprot.org/uniprot/#_A2AQB2-mappedCitation-18434434 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18434434 |
| http://purl.uniprot.org/uniprot/#_A0A571BF46-mappedCitation-18434434 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18434434 |
| http://purl.uniprot.org/uniprot/#_A0A571BF58-mappedCitation-18434434 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18434434 |
| http://purl.uniprot.org/uniprot/#_A0A1B0GSF5-mappedCitation-18434434 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18434434 |
| http://purl.uniprot.org/uniprot/#_A0A1B0GSI0-mappedCitation-18434434 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18434434 |