| http://purl.uniprot.org/citations/18440551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18440551 | http://www.w3.org/2000/01/rdf-schema#comment | "Guanine nucleotide exchange factors (GEFs) regulate the activity of small G proteins by catalysing the intrinsically slow exchange of GDP for GTP. The mechanism involves the formation of trimeric G protein-nucleotide-GEF complexes, followed by the release of nucleotide to form stable binary G protein-GEF complexes. A number of structural studies of G protein-GEF complexes have shown large structural changes induced in the nucleotide binding site. Together with a recent structure of a trimeric complex, these studies have suggested not only some common principles but also large differences in detail in the GEF-mediated exchange reaction. Several structures suggested that a glutamic acid residue in switch II, which is part of the DxxGQE motif and highly conserved in Ras-like G proteins, might have a decisive mechanistic role in GEF-mediated nucleotide exchange reactions. Here we show that mutation of the switch II glutamate to Ala severely impairs GEF-catalysed nucleotide exchange in most, but not all, Ras family G proteins, explaining its high sequence conservation. The residue determines the initial approach of GEF to the nucleotide-loaded G protein and does not appreciably affect the formation of a binary nucleotide-free complex. Its major effect thus appears to be the removal of the P-loop lysine from its interaction with the nucleotide."xsd:string |
| http://purl.uniprot.org/citations/18440551 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2008.03.011"xsd:string |
| http://purl.uniprot.org/citations/18440551 | http://purl.uniprot.org/core/author | "Thomas C."xsd:string |
| http://purl.uniprot.org/citations/18440551 | http://purl.uniprot.org/core/author | "Wittinghofer A."xsd:string |
| http://purl.uniprot.org/citations/18440551 | http://purl.uniprot.org/core/author | "Gasper R."xsd:string |
| http://purl.uniprot.org/citations/18440551 | http://purl.uniprot.org/core/author | "Ahmadian M.R."xsd:string |
| http://purl.uniprot.org/citations/18440551 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18440551 | http://purl.uniprot.org/core/name | "J Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/18440551 | http://purl.uniprot.org/core/pages | "51-63"xsd:string |
| http://purl.uniprot.org/citations/18440551 | http://purl.uniprot.org/core/title | "The role of the conserved switch II glutamate in guanine nucleotide exchange factor-mediated nucleotide exchange of GTP-binding proteins."xsd:string |
| http://purl.uniprot.org/citations/18440551 | http://purl.uniprot.org/core/volume | "379"xsd:string |
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