http://purl.uniprot.org/citations/18441218 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/18441218 | http://www.w3.org/2000/01/rdf-schema#comment | "NADPH:protochlorophyllide (Pchlide) oxidoreductase (POR) A is the only known example thus far of a nucleus-encoded plastid protein that is imported to its final destination in a substrate-dependent, Pchlide-regulated manner. Previous work has shown that the cytosolic PORA precursor (pPORA) does not utilize the general import site but uses a distinct translocon designated the Pchlide-dependent translocon complex. Here we demonstrate that a pentapeptide motif, threonine-threonine-serine-proline-glycine (TTSPG) in pPORA's transit peptide (transA), is involved in Pchlide-dependent transport. Deletion of this motif from the COOH-terminal end of transA abolished both Pchlide binding and protein import. Incorporation of the TTSPG motif into normally non-Pchlide-responsive transit sequences conferred the pigment binding properties onto the engineered chimeric precursors but was insufficient to render protein import substrate dependent. An additional motif was identified in the NH(2)-terminal part of transA that was needed for binding of the precursor to the Pchlide-dependent translocon complex. Point mutations of the TTSPG motif identified glycine as the Pchlide binding site. By analogy to the major light-harvesting chlorophyll a/b binding protein of photosystem II, we propose that the peptidyl carbonyl oxygen of glycine may bind directly or via a water molecule to the central Mg atom of the pigment."xsd:string |
http://purl.uniprot.org/citations/18441218 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.108.120113"xsd:string |
http://purl.uniprot.org/citations/18441218 | http://purl.uniprot.org/core/author | "Quigley F."xsd:string |
http://purl.uniprot.org/citations/18441218 | http://purl.uniprot.org/core/author | "Pollmann S."xsd:string |
http://purl.uniprot.org/citations/18441218 | http://purl.uniprot.org/core/author | "Weisbeek P."xsd:string |
http://purl.uniprot.org/citations/18441218 | http://purl.uniprot.org/core/author | "Reinbothe C."xsd:string |
http://purl.uniprot.org/citations/18441218 | http://purl.uniprot.org/core/author | "Reinbothe S."xsd:string |
http://purl.uniprot.org/citations/18441218 | http://purl.uniprot.org/core/author | "Phetsarath-Faure P."xsd:string |
http://purl.uniprot.org/citations/18441218 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
http://purl.uniprot.org/citations/18441218 | http://purl.uniprot.org/core/name | "Plant Physiol"xsd:string |
http://purl.uniprot.org/citations/18441218 | http://purl.uniprot.org/core/pages | "694-703"xsd:string |
http://purl.uniprot.org/citations/18441218 | http://purl.uniprot.org/core/title | "A pentapeptide motif related to a pigment binding site in the major light-harvesting protein of photosystem II, LHCII, governs substrate-dependent plastid import of NADPH:protochlorophyllide oxidoreductase A."xsd:string |
http://purl.uniprot.org/citations/18441218 | http://purl.uniprot.org/core/volume | "148"xsd:string |
http://purl.uniprot.org/citations/18441218 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18441218 |
http://purl.uniprot.org/citations/18441218 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18441218 |
http://purl.uniprot.org/uniprot/#_F4I2F8-mappedCitation-18441218 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18441218 |
http://purl.uniprot.org/uniprot/#_Q0WVW0-mappedCitation-18441218 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18441218 |
http://purl.uniprot.org/uniprot/#_O48741-mappedCitation-18441218 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18441218 |
http://purl.uniprot.org/uniprot/#_Q42536-mappedCitation-18441218 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18441218 |
http://purl.uniprot.org/uniprot/Q42536 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18441218 |
http://purl.uniprot.org/uniprot/F4I2F8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18441218 |
http://purl.uniprot.org/uniprot/O48741 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18441218 |
http://purl.uniprot.org/uniprot/Q0WVW0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18441218 |