| http://purl.uniprot.org/citations/18442887 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18442887 | http://www.w3.org/2000/01/rdf-schema#comment | "Little is known about the upstream regulator of Runx2, a master regulator of osteoblast differentiation in bone tissues. To elucidate the molecular mechanism of Runx2 gene expression, we analyzed Runx2 promoter activity in osseous (MC3T3-E1, KS483, Kusa) and non-osseous (NIH3T3, C3H10T1/2, mouse embryonic fibroblasts) cells and also identified Runx2 upstream regulator using a Runx2 promoter-derived luciferase reporter system. After cloning 15 serial deletion constructs from -6832 bp/+390 bp to -37 bp/+390 bp of the Runx2-P1 promoter, we performed a transient transfection assay in osseous and non-osseous cells. A reduction in Runx2 promoter activity was observed in two regions; one was between -3 kb and -1 kb, and the other was between -155 bp and -75 bp. The step-down pattern in promoter activity between -3 kb and -1 kb was observed only in osseous cells. Interestingly, the step-down pattern between -155 bp and -75 bp was revealed in both cell types. Consistently, beta-galactosidase staining in axial skeleton of -3 kb-Runx2-P1-LacZ transgenic mice was positive, but that of all skeletal tissues of -1 kb-Runx2-P1-LacZ transgenic mice was negative. To identify upstream regulators of the Runx2-P1 promoter, we screened 100 transcription factors using Runx2-P1-luciferase reporter constructs in NIH3T3 fibroblasts and HeLa cells. Among them, HIF2A was identified as the strongest activator of Runx2-P1 promoter activity. A HIF2A-responsive site on the Runx2 promoter was identified between -106 bp and -104 bp by mutation analysis. An electrophoretic mobility shift assay and chromatin immunoprecipitation assay confirmed the binding of HIF2A to the Runx2-P1 promoter in vitro and in vivo, respectively. Knock-down using siRNA against HIF2A confirmed that HIF2A is an important regulator of Runx2 gene expression. Collectively, these results suggest that the region between -3 kb and -1 kb is required for the minimal skeletal tissue-specific expression of Runx2, and that the region between -155 bp and -75 bp is important for its basal transcription, which may be in part mediated by HIF2A in bone tissues."xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.gene.2008.03.003"xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/author | "Kawaguchi H."xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/author | "Ikeda T."xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/author | "Choi J.Y."xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/author | "Saito T."xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/author | "Tamiya H."xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/author | "Jeong J.H."xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/author | "Jung Y.K."xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/author | "Ohba S."xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/author | "Chung U.I."xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/author | "Yano F."xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/pages | "53-60"xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/title | "Analysis of the Runx2 promoter in osseous and non-osseous cells and identification of HIF2A as a potent transcription activator."xsd:string |
| http://purl.uniprot.org/citations/18442887 | http://purl.uniprot.org/core/volume | "416"xsd:string |
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| http://purl.uniprot.org/uniprot/#_F7CN98-mappedCitation-18442887 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18442887 |